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논문 기본 정보

자료유형
학술저널
저자정보
Kim Jeongtae (Department of Electronic Engineering Hanbat National University Daejeon 34158 Republic of Korea) Lee Jun Bong (College of Veterinary Medicine & Institute of Veterinary Science Kangwon National University Gangwon 24341 Republic of Korea) Park Jaewon (Green Manufacturing Research Center Korea University Seoul 02841 Republic of Korea) Koo Chiwan (Department of Electronic Engineering Hanbat National University Daejeon 34158 Republic of Korea) Lee Moo-Seung (Environmental Diseases Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon 34141 Republic of KoreaDepartment of Biomolecular Science KRIBB School of Bioscience Korea Unive)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제33권 제5호
발행연도
2023.5
수록면
559 - 573 (15page)
DOI
10.4014/jmb.2212.12025

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Shiga toxin (Stxs)-producing enterohaemorrhagic Escherichia coli (EHEC) and Shigella dysenteriae serotype 1 are major causative agents of severe bloody diarrhea (known as hemorrhagic colitis) and hemolytic uremic syndrome (HUS) associated with extraintestinal complications such as acute renal failure and neurologic impairment in infected patients under 9 years of age. Extreme nephrotoxicity of Stxs in HUS patients is associated with severe outcomes, highlighting the need to develop technologies to detect low levels of the toxin in environmental or food samples. Currently, the conventional polymerase chain reaction (PCR) or immunoassay is the most broadly used assay to detect the toxin. However, these assays are laborious, time-consuming, and costly. More recently, numerous studies have described novel, highly sensitive, and portable methods for detecting Stxs from EHEC. To contextualize newly emerging Stxs detection methods, we briefly explain the basic principles of these methods, including lateral flow assays, optical detection, and electrical detection. We subsequently describe existing and newly emerging rapid detection technologies to identify and measure Stxs.

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