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학술저널
저자정보
Min Jung Kim (Kyung Hee University) Heejin Lim (Daegu Gyeongbuk Institute of Science and Technology (DGIST)) Muwoong Kim (Kyung Hee University) Yuri Choi (Kyung Hee University) Thy N. C. Nguyen (Daegu Gyeongbuk Institute of Science and Technology (DGIST)) Seung Cheol Park (Kyung Hee University) Kwang Pyo Kim (Kyung Hee University) Junyang Jung (Kyung Hee University) Min-Sik Kim (Daegu Gyeongbuk Institute of Science and Technology (DGIST))
저널정보
한국질량분석학회 Mass Spectrometry Letters Mass Spectrometry Letters Vol.13 No.2
발행연도
2022.6
수록면
35 - 48 (14page)

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Human tissues and organs can be preserved intact by fixation with formalin for the future analysis of biomolecules of interest. With the advances in high-throughput methods, numerous protocols have been developed and optimized to attain the most pathophysiological information out of biomolecules, including RNA and proteins, in formalin-fixed samples. However, there is no systematic study to examine the effects of formalin fixation on the lipidome of biological samples in a global fashion. In this study, we conducted a mass spectrometry-based analysis to survey the alteration in the lipidome of mice brains by fixation methods. A total of 308 lipids were quantitatively measured using triple quadrupole mass spectrometry. We found that most were unchanged after formalin fixation except for a few lipid classes such as phosphatidylethanolamine.

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Abstract
Introduction
Results and Discussion
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