지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
J. A. Atkinson / 2017 / An updated protocol for high throughput plant tissue sectioning / Front. Plant Sci 8:1721
H. Bargel / 2005 / Tomato (Lycopersicon esculentum mill.) fruit growth and ripening as related to the biomechanical properties of fruit skin and isolated cuticle / J. Expt. Bot 56:1049~1060
C. Chieco / 2012 / An ethanol-based fixation method for anatomical and micro-morphological characterization of leaves of various tree species / Biotech. Histochem 88:109~119
K. Das / 2008 / Methane emission associated with anatomical and morphophysiological characteristics of rice(Oryza sativa)plant / Physiol. Planta 134:303~312
H. G. Edelmann / 1995 / Characterization of hydration-dependent wall-extensible properties of rye coleoptiles : Evidence for auxin-induced changes of hydrogen bonding / J. Plant Physiol 145:491~497
H. Bargel / 2005 / Tomato (Lycopersicon esculentum mill.) fruit growth and ripening as related to the biomechanical properties of fruit skin and isolated cuticle / J. Expt. Bot 56:1049~1060
C. Chieco / 2012 / An ethanol-based fixation method for anatomical and micro-morphological characterization of leaves of various tree species / Biotech. Histochem 88:109~119
K. Das / 2008 / Methane emission associated with anatomical and morphophysiological characteristics of rice(Oryza sativa)plant / Physiol. Planta 134:303~312
H. G. Edelmann / 1995 / Characterization of hydration-dependent wall-extensible properties of rye coleoptiles : Evidence for auxin-induced changes of hydrogen bonding / J. Plant Physiol 145:491~497
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