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논문 기본 정보

자료유형
학술저널
저자정보
Hayoung Yang (Chonnam National University) Woosun Kim (Chonnam National University) Junwoo Bae (Chonnam National University) Hyunwoo Kim (Chonnam National University) Sangki Kim (Kongju National University) Jihye Choi (Chonnam National University) Jinho Park (Chonbuk National University) Dong-In Jung (Gyeongsang National University) HongBum Koh (Chonnam National University) DoHyeon Yu (Gyeongsang National University)
저널정보
대한수의학회 Journal of Veterinary Science Journal of Veterinary Science 제20권 제1호
발행연도
2019.1
수록면
72 - 78 (7page)

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White blood cells (WBCs) and storage period are the main factors of transfusion reactions. In the present study, cytokine/chemokine concentrations after leukoreduction (LR) and irradiation (IR) in stored canine whole blood were measured. Red blood cell storage lesion caused by IR and LR were also compared. Blood samples from 10 healthy Beagles were divided into four groups (no treatment, LR-, IR-, and LR+ IR-treated). Leukocytes were removed by filtration in the LR group and gamma radiation (25 Gy) was applied in he IR group. Immunologic factors (WBCs, interleukin-6 [IL-6], C-X-C motif chemokine ligand 8 [CXCL-8], and tumor necrosis factor-alpha) and storage lesion factors (blood pH, potassium, and hemolysis) were evaluated on storage days 0, 7, 14, 21, and 28. Compared to the treated groups, IL-6 and CXCL-8 concentrations during storage were significantly higher in the control (no treatment) group. LR did not show changes in cytokine/chemokine concentrations, and storage lesion presence was relatively mild. IR significantly increased CXCL-8 after 14 days of storage, but IR of leukoreduced blood did not increase CXCL-8 during 28 days of storage. Storage lesions such as hemolysis, increased potassium, and low pH were observed 7 days after IR and storage of blood, regardless of LR. IR of leukoreduced blood is beneficial to avoid immune reactions; however, storage lesions should be considered upon storage.

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Introduction
Materials and Methods
Results
Discussion
References

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