Identification and Functional Analysis of RelA/SpoT Homolog (RSH) Genes in Deinococcus radiodurans

Jinhui Wang; Ye Tian; Zhengfu Zhou; Liwen Zhang; Wei Zhang; Min Lin; Ming Chen

doi:10.4014/jmb.1601.01017

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자료유형: 학술저널

저자정보: Jinhui Wang (Chinese Academy of Agricultural Sciences) Ye Tian (Chinese Academy of Agricultural Sciences) Zhengfu Zhou (Chinese Academy of Agricultural Sciences) Liwen Zhang (Chinese Academy of Agricultural Sciences) Wei Zhang (Chinese Academy of Agricultural Sciences) Min Lin (Chinese Academy of Agricultural Sciences) Ming Chen (Chinese Academy of Agricultural Sciences)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제12호

발행연도: 2016.12

수록면: 2,106 - 2,115 (10page)

DOI: 10.4014/jmb.1601.01017

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To identify the global effects of (p)ppGpp in the gram-positive bacterium Deinococcus radiodurans, which exhibits remarkable resistance to radiation and other stresses, RelA/SpoT homolog (RSHs) mutants were constructed by direct deletion mutagenesis. The results showed that RelA has both synthesis and hydrolysis domains of (p)ppGpp, whereas RelQ only synthesizes (p)ppGpp in D. radiodurans. The growth assay for mutants and complementation analysis revealed that deletion of relA and relQ sensitized the cells to H2O2, heat shock, and amino acid limitation. Comparative proteomic analysis revealed that the bifunctional RelA is involved in DNA repair, molecular chaperone functions, transcription, the tricarboxylic acid cycle, and metabolism, suggesting that relA maintains the cellular (p)ppGpp levels and plays a crucial role in oxidative resistance in D. radiodurans. The D. radiodurans relA and relQ genes are responsible for (p)ppGpp synthesis/hydrolysis and (p)ppGpp hydrolysis, respectively. (p)ppGpp integrates a general stress response with a targeted re-programming of gene regulation to allow bacteria to respond appropriately towards heat shock, oxidative stress, and starvation. This is the first identification of RelA and RelQ involvement in response to oxidative, heat shock, and starvation stresses in D. radiodurans, which further elucidates the remarkable resistance of this bacterium to stresses.

#RelA #RelQ #(p)ppGpp #oxidative stress #Deinococcus radiodurans #RelA/SpoT homolog

참고문헌 (54)

참고문헌 신청

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