Equol Induces Mitochondria-Dependent Apoptosis in Human Gastric Cancer Cells via the Sustained Activation of ERK1/2 Pathway

Xiaonan Wu; Zhiping Yang; Yan Zhao; Yahong Yao; Jun Li; Wangshi Wang

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자료유형: 학술저널

저자정보: Xiaonan Wu (Xiamen Medical College) Zhiping Yang (The First Affiliated Hospital of Xiamen) Yan Zhao (The First Affiliated Hospital of Xiamen) Yahong Yao (The First Affiliated Hospital of Xiamen) Jun Li (The First Affiliated Hospital of Xiamen) Wangshi Wang (The First Affiliated Hospital of Xiamen)

저널정보: 한국분자세포생물학회 Molecules and Cells Molecules and Cells 제39권 제10호

발행연도: 2016.10

수록면: 742 - 749 (8page)

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The cancer chemo-preventive effects of equol have been demonstrated for a wide variety of experimental tumours. In a previous study, we found that equol inhibited proliferation and induced apoptotic death of human gastric cancer MGC-803 cells. However, the mechanisms underlying equol-mediated apoptosis have not been well understood. In the present study, the dual AO (acridine orange)/EB (ethidium bromide) fluorescent assay, the comet assay, MTS, western blotting and flow cytometric assays were performed to further investigate the pro-apoptotic effect of equol and its associated mechanisms in MGC-803 cells. The results demonstrated that equol induced an apoptotic nuclear morphology revealed by AO/EB staining, the presence of a comet tail, the cleavage of caspase-3 and PARP and the depletion of cIAP1, indicating its pro-apoptotic effect. In addition, equol-induced apoptosis involves the mitochondria-dependent cell-death pathway, evidenced by the depolarization of the mitochondrial membrane potential, the cleavage of caspase-9 and the depletion of Bcl-xL and full-length Bid. Moreover, treating MGC-803 cells with equol induced the sustained activation of extracellular signal-regulated kinase (ERK), and inhibiting ERK by U0126, a MEK/ERK pathway inhibitor, significantly attenuated the equol-induced cell apoptosis. These results suggest that equol induces mitochondria-dependent apoptosis in human gastric cancer MGC-803 cells via the sustained activation of the ERK1/2 pathway. Therefore, equol may be a novel candidate for the chemoprevention and therapy of gastric cancer.

#ERK signalling pathway #equol #gastric cancer #mitochondria-dependent apoptosis

참고문헌 (30)

참고문헌 신청

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Xiaonan Wu

소속기관 Xiamen Medical College

주요연구분야 자연과학 > 생물학

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Zhiping Yang