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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.3
- 수록면
- 113 - 120 (8page)
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A main symptom of equine metabolic syndrome (EMS) in ponies is pathological obesity characterized by abnormal accumulation of fatdeposits and inflammation. In this study, we analyzed the expression of two pro-inflammatory cytokines, interleukin-6 (IL-6) and tumornecrosis factor-alpha (TNF-α), in subcutaneous adipose tissue and the correlation with serum concentrations in peripheral blood of Welshponies. Based on clinical examination findings, the animals were divided into two groups: ponies affected with EMS (n = 8) and obese ponies(n = 8). The adipose tissue was examined using immunohistochemical analysis while concentrations IL-6 and TNF-αwere measured usingenzyme-linked immunosorbent assays (ELISAs). Additionally, histological characterization of the adipose tissue was performed. The resultsobtained showed that IL-6 expression in adipose tissue biopsies derived from animals with EMS was enhanced while TNF-αlevels of bothgroups were comparable. Compared to the obese ponies, EMS animals also had significantly elevated levels of serum IL-6 and TNF-α.Histological analysis revealed macrophage infiltration and fibrosis in adipose tissue preparations from the EMS group. These data suggestthat IL-6 may play a key role in the course of EMS in Welsh ponies. Our findings also demonstrated that analysis of pro-inflammatory cytokineslevels in serum may serve as an additional tool for diagnosing EMS.
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