In vitro regeneration of Phaseolus vulgaris L. via direct and indirect organogenesis

Yu Yan; Liu Dajun; Liu Chang; Yan Zhishan; Yang Xiaoxu; Feng Guojun

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자료유형: 학술저널

저자정보: Yu Yan (Heilongjiang University) Liu Dajun (Heilongjiang University) Liu Chang (Heilongjiang University) Yan Zhishan (Heilongjiang University) Yang Xiaoxu (Heilongjiang University) Feng Guojun (Heilongjiang University)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제15권 제3호

발행연도: 2021.1

수록면: 279 - 288 (10page)

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A stable and efcient regeneration system is a necessary condition for the genetic transformation of Phaseolus vulgaris L. In this study, cotyledonary nodes with one cotyledon, cotyledonary nodes with two cotyledons, embryonic axes and root segments of 5-day-old germinated seeds were used as explants to evaluate explants to induce shoots and calli. Besides, using cotyledonary nodes with one cotyledon as explants, the efects of diferent concentrations of auxins and cytokinins on the direct and indirect organogenesis of common bean were investigated. As a result, embryonic axes and cotyledonary nodes with two cotyledons were the most efective explant types for the formation of shoots and calli. The cotyledonary nodes with one cotyledon were cultured in the shoot induction medium containing 7?mg?L?1 6-benzyladenine (BAP) and 0.2?mg?L?1 α-naphthaleneacetic acid (NAA). 74.21% of explants directly formed shoots and average number of shoots was up to 2.91. On the rooting medium supplemented with 0.5?mg?L?1 indole-3-butyric acid (IBA), the rooting rate of shoots reached 100% and the number of main roots formed was the most. The addition of 0.5?mg?L?1 thidiazuron (TDZ) was optimum for callus induction, and the induction rate could reach 91.51%. The shoot regeneration frequency was 93.33% when calli subcultured on the shoot induction medium supplemented with 1.0?mg?L?1 BAP. Average 4.27 shoots were produced by per callus and rooted on the shoot induction medium. This report descripts an efcient common bean regeneration system through direct and indirect organogenesis, which can provide reference for its subsequent genetic transformation research.

#Direct organogenesis?· Indirect organogenesis?· Phaseolus vulgaris L.?· Regeneration

참고문헌 (29)

참고문헌 신청

Ahmed EE / 2002 / Plant regeneration from seedling explants of common bean (Phaseolous vulgaris L.) / Acta Biol Szeged 46:27~28

Arellano J / 2009 / Regeneration of different cultivars of common bean (Phaseolus vulgaris L.) via indirect organogenesis / Plant Cell Tissue Organ Cult 96:11~18

Assefa T / 2019 / A review of breeding objectives, genomic resources, and marker-assisted methods in common bean (Phaseolus vulgaris L.) / Mol Breed 39:20

Beebe SE / 2013 / Phenotyping common beans for adaptation to drought / Front Physiol 4:35

Benedičič DRM / 1997 / The regeneration of bean plants from meristem culture / Phyton Ann Rei Bot A 37:151~160

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