지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
이용수
초록· 키워드
오류제보하기
In Comamonas sp. strain P19, homogentisate 1,2-dioxygenase (HGO) catalyzes the conversion of homogentisate to4-maleylacetoacetate by aromatic ring scission, in the breakdown of tyrosine and phenylalanine. To determine themolecular background of the enzymatic mechanism of HGO in this zinc-resistant organism, hmgA encoding HGO ofComamonas sp. strain P19 was cloned, and the expressed protein was purified. The protein was crystallized in solutions I[25% (w/v) polyethylene glycol 3350 and 0.1 M trisodium citrate at pH 5.6] and II [1.4 M ammonium tartrate and 0.1 M bis-Tris at pH 5.5]. X-ray diffraction data were collected to 1.8 A resolution using synchrotron radiation. The crystal belongs tothe orthorhombic space group P212121, with unit cell dimensions of a = 73.2 A, b = 100.0 A, and c = 134.9 A. A traceableelectron density map was calculated using anomalous diffraction data obtained from a crystal soaked in zinc ions.
목차
등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
Adams, P. D / 2010 / PHENIX : a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr D Biol Crystallogr 66:213~221
Borowski, T / 2005 / Catalytic reaction mechanism of homogentisate dioxygenase : a hybrid DFT study / J Am Chem Soc 127:17303~17314
Fernandez-Canon, J. M / 1996 / The molecular basis of alkaptonuria / Nat Genet 14:19~24
Jeoung, J. H / 2013 / Visualizing the substrate-, superoxo-, alkylperoxo-, and product-bound states at the nonheme Fe(II)site of homogentisate dioxygenase / Proc Natl Acad Sci U S A 110:12625~12630
Knox, W. E / 1955 / Homogentisate oxidase of liver / J Biol Chem 216:479~487
Borowski, T / 2005 / Catalytic reaction mechanism of homogentisate dioxygenase : a hybrid DFT study / J Am Chem Soc 127:17303~17314
Fernandez-Canon, J. M / 1996 / The molecular basis of alkaptonuria / Nat Genet 14:19~24
Jeoung, J. H / 2013 / Visualizing the substrate-, superoxo-, alkylperoxo-, and product-bound states at the nonheme Fe(II)site of homogentisate dioxygenase / Proc Natl Acad Sci U S A 110:12625~12630
Knox, W. E / 1955 / Homogentisate oxidase of liver / J Biol Chem 216:479~487
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Phase Determination of Iron-dependent Homogentisate Dioxygenase from Acinetobacter oleivorans
한국미생물학회 학술대회논문집
2017 .04
-
Crystal structure analysis using X-ray Diffraction
한국분석과학회 학술대회
2017 .05
-
Complete genome sequence of Comamonas sp. NLF-7-7 isolated from biofilter of wastewater treatment plant
미생물학회지
2019 .09
-
Catabolism of 2-Hydroxyphenylacetate in Comamonas testosteroni Strain P19
한국미생물학회 학술대회논문집
2020 .10
-
Identification of a Gene Involved in the Negative Regulation of Pyomelanin Production in Ralstonia solanacearum
Journal of Microbiology and Biotechnology
2017 .01
-
섬유 폐수 활성 슬러지에서 분리한 Comamonas testosteroni의 생물학적 페놀 분해
생명과학회지
2020 .02
-
Crystal structural analysis of InAsxP1-x nanowire exploiting high resolution X-ray diffraction
한국진공학회 학술발표회초록집
2017 .08
-
Lysobacter sp. Strain II4 Isolated from Soil
한국미생물학회 학술대회논문집
2019 .04
-
조팝나무(Spiraea prunifolia var. simpliciflora) 에탄올 추출물 분획물의 항산화 활성
한국생활과학회지
2019 .12
-
Isolation and Characterization of a Comamonas thiooxydans N1 Strain able to Degrade the Fungicide Biphenyl of Control of Postharvest Agricultural Products
한국미생물학회 학술대회논문집
2022 .10
-
Biodegradation of Diesel Oil and n-Alkanes (C18, C20, and C22) by Novel Strain Acinetobacter sp. K-6 in Unsaturated Soil
한국미생물학회 학술대회논문집
2019 .04
-
Structural studies of rechargeable batteries and perovskite materials using in-situ synchrotron X-ray scattering/diffraction techniques
한국진공학회 학술발표회초록집
2020 .02
-
Soft continuity and SP-continuity
ANNALS OF FUZZY MATHEMATICS AND INFORMATICS
2020 .01
-
Demethylation of Vanillate and 4-Methoxybenzoate in Comamonas testosteroni Strain P19
한국미생물학회 학술대회논문집
2021 .08
-
New species and records of the spider families Pholcidae, Uloboridae, Linyphiidae, Theridiidae, Phrurolithidae, and Thomisidae (Araneae) from Korea
Journal of species research
2018 .01
-
Characterization of Flavobacterium sp. Strain I3-3 Isolated from River Water
한국미생물학회 학술대회논문집
2017 .04
-
Transcriptomic Identification and Biochemical Characterization of HmpA, a Nitric Oxide Dioxygenase, Essential for Pathogenesis of Vibrio vulnificus
한국미생물학회 학술대회논문집
2019 .04
-
A report on 15 unrecorded bacterial species of Korea isolated in 2016, belonging to the class Betaproteobacteria
Journal of species research
2018 .01
-
Purification and preliminary X-ray analysis of YbeY from Staphylococcus aureus
Biodesign
2021 .01
-
Report of 21 unrecorded bacterial species in Korea belonging to Betaproteobacteria and Epsilonproteobacteria
Journal of species research
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0