지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Endoribonuclease YbeY is essential for maturing ribosomal RNA in a wide range of bacteria. YbeY is specific to the singlestranded RNA of ribosomal RNAs and small RNAs. However, the structural relationship with the function and recognition of substrates remains elucidated. In this study, we overexpressed full-length YbeY from the gram-positive bacterium Staphylococcus aureus. The protein was purified and crystallized under conditions containing 0.1 M sodium citrate (pH 5.0) and 44% (w/v) polyethylene glycol 600. An X-ray diffraction dataset at a resolution of 1.9 A was collected, and the dataset belonged to space group P63 with unit cell parameters a = 100.7 and c = 50.2 A. The single-wavelength anomalous diffraction method was successful in determining the crystal structure. We are now refining the structure combined with structural analysis. The structure will provide molecular insights into the substrate recognition mechanism by showing the high-resolution features of the active sites.
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참고문헌 (21)
참고문헌 신청
Adams, P. D. / 2010 / PHENIX : a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr D Biol Crystallogr 66:213~221
Afonine, P. V. / 2012 / Towards automated crystallographic structure refinement with phenix. refine / Acta Crystallogr D Biol Crystallogr 68:352~367
Afonine, P. V. / 2010 / Joint X-ray and neutron refinement with phenix. refine / Acta Crystallogr D Biol Crystallogr 66:1153~1163
Babu, V. M. P. / 2020 / Sinorhizobium meliloti YbeY is a zinc-dependent single-strand specific endoribonuclease that plays an important role in 16S ribosomal RNA processing / Nucleic Acids Res 48:332~348
Butland, G. / 2005 / Interaction network containing conserved and essential protein complexes in Escherichia coli / Nature 433:531~537
Afonine, P. V. / 2012 / Towards automated crystallographic structure refinement with phenix. refine / Acta Crystallogr D Biol Crystallogr 68:352~367
Afonine, P. V. / 2010 / Joint X-ray and neutron refinement with phenix. refine / Acta Crystallogr D Biol Crystallogr 66:1153~1163
Babu, V. M. P. / 2020 / Sinorhizobium meliloti YbeY is a zinc-dependent single-strand specific endoribonuclease that plays an important role in 16S ribosomal RNA processing / Nucleic Acids Res 48:332~348
Butland, G. / 2005 / Interaction network containing conserved and essential protein complexes in Escherichia coli / Nature 433:531~537
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