지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 975 - 988 (14page)
- DOI
- 10.1007/s13770-021-00372-x
이용수
초록· 키워드
오류제보하기
Background: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a severe sequela caused by bisphosphonates (BPs), which are widely used to treat osteoporosis or other malignancies. However, the mechanism underlying BRONJ remains unclear. Recently, human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have been studied for treatment of diverse diseases and injuries. This study aimed to investigate the therapeutic effects of hUC-MSCs in BRONJ.
Methods: The therapeutic effects of hUC-MSCs were examined in rat bone marrow (rBM)-derived cells using cell viability, colony-forming, and real-time PCR assays and FACS for analyzing essential proinflammatory and bone regeneration markers in vitro. To demonstrate the in vivo therapeutic and adverse effects of transfused hUC-MSCs, micro-CT, H&E staining, IHC (Angiogenesis marker gene expression) staining, and parathyroid hormone (PTH)/calcium assay were conducted in a BRONJ-induced animal model.
Results: BP-induced cytotoxicity and inflammation in rBM-derived cells decreased, after co-culture with hUC-MSCs. The expression levels of bone regeneration markers (RUNX2, OSX, and BMP-2) significantly increased in BP-treated rBM-derived cells, after co-culture with hUC-MSCs. The BP-induced abnormal shift in RANKL/OPG expression ratio in rBM-derived cells was normalized by hUC-MSCs. Consistent with these in vitro results, transfused hUC-MSCs markedly decreased BRONJ and significantly healed injured mucosa in the BRONJ-induced animal model. The animals exhibited serious destruction of the kidney structure and increases in serum PTH and calcium levels, which were significantly normalized by hUC-MSC transfusion.
Conclusion: hUC-MSCs exerted therapeutic effects on BRONJ in vitro and in vivo through their anti-cytotoxicity, anti-inflammatory activity and ability to recover bone regeneration.
목차
등록된 정보가 없습니다.
참고문헌 (44)
참고문헌 신청
Kim J / 2020 / Bisphosphonate-related osteonecrosis of the jaw : current clinical significance and treatment strategy review / Am J Dent 33:115~128
Silverman SL / 2009 / Osteonecrosis of the jaw and the role of bisphosphonates: a critical review / Am J Med 122:S33~S45
Bagan J / 2009 / Osteonecrosis of the jaws in patients treated with intravenous bisphosphonates(BRONJ) : a concise update / Oral Oncol 45:551~554
Agrillo A / 2012 / Bisphosphonate-related osteonecrosis of the jaw(BRONJ) : 5 year experience in the treatment of 131 cases with ozone therapy / Eur Rev Med Pharmacol Sci 16:1741~1747
Fliefel R / 2015 / Treatment strategies and outcomes of bisphosphonate-related osteonecrosis of the jaw(BRONJ)with characterization of patients : a systematic review / Int J Oral Maxillofac Surg 44:568~585
Silverman SL / 2009 / Osteonecrosis of the jaw and the role of bisphosphonates: a critical review / Am J Med 122:S33~S45
Bagan J / 2009 / Osteonecrosis of the jaws in patients treated with intravenous bisphosphonates(BRONJ) : a concise update / Oral Oncol 45:551~554
Agrillo A / 2012 / Bisphosphonate-related osteonecrosis of the jaw(BRONJ) : 5 year experience in the treatment of 131 cases with ozone therapy / Eur Rev Med Pharmacol Sci 16:1741~1747
Fliefel R / 2015 / Treatment strategies and outcomes of bisphosphonate-related osteonecrosis of the jaw(BRONJ)with characterization of patients : a systematic review / Int J Oral Maxillofac Surg 44:568~585
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