Biomimetic Biphasic Electrospun Scaffold for Anterior Cruciate Ligament Tissue Engineering

Tang Ya; Tian Jialiang; Li Long; Huang Lin; Shen Quan; Guo Shanzhu; Jiang Yue

doi:10.1007/s13770-021-00376-7

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저자정보: Tang Ya (Orthopedic Department Guizhou Provincial People’s Hospital) Tian Jialiang (Orthopedic Department Guizhou Provincial People’s Hospital) Li Long (College of Materials and Metallurgy Guizhou University) Huang Lin (College of Materials and Metallurgy Guizhou University) Shen Quan (College of Materials and Metallurgy Guizhou Unive) Guo Shanzhu (College of Materials and Metallurgy Guizhou University) Jiang Yue (College of Materials and Metallurgy Guizhou University)

저널정보: 한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제18권 제5호

발행연도: 2021.10

수록면: 819 - 830 (12page)

DOI: 10.1007/s13770-021-00376-7

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Background: Replacing damaged anterior cruciate ligaments (ACLs) with tissue-engineered artificial ligaments is challenging because ligament scaffolds must have a multiregional structure that can guide stem cell differentiation. Here, we designed a biphasic scaffold and evaluated its effect on human marrow mesenchymal stem cells (MSCs) under dynamic culture conditions as well as rat ACL reconstruction model in vivo. Methods: We designed a novel dual-phase electrospinning strategy wherein the scaffolds comprised randomly arranged phases at the two ends and an aligned phase in the middle. The morphological, mechanical properties and scaffold degradation were investigated. MSCs proliferation, adhesion, morphology and fibroblast markers were evaluated under dynamic culturing. This scaffold were tested if they could induce ligament formation using a rodent model in vivo. Results: Compared with other materials, poly(D,L-lactide-co-glycolide)/poly(ε-caprolactone) (PLGA/PCL) with mass ratio of 1:5 showed appropriate mechanical properties and biodegradability that matched ACLs. After 28 days of dynamic culturing, MSCs were fusiform oriented in the aligned phase and randomly arranged in a paving-stone-like morphology in the random phase. The increased expression of fibroblastic markers demonstrated that only the alignment of nanofibers worked with mechanical stimulation to promote effective fibroblast differentiation. This scaffold was a dense collagenous structure, and there was minimal difference in collagen direction in the orientation phase. Conclusion: Dual-phase electrospun scaffolds had mechanical properties and degradability similar to those of ACLs. They promoted differences in the morphology of MSCs and induced fibroblast differentiation under dynamic culture conditions. Animal experiments showed that ligamentous tissue regenerated well and supported joint stability.

Background: Replacing damaged anterior cruciate ligaments (ACLs) with tissue-engineered artificial ligaments is challenging because ligament scaffolds must have a multiregional structure that can guide stem cell differentiation. Here, we designed a biphasic scaffold and evaluated its effect on human marrow mesenchymal stem cells (MSCs) under dynamic culture conditions as well as rat ACL reconstruction model in vivo. Methods: We designed a novel dual-phase electrospinning strategy wherein the scaffolds comprised randomly arranged phases at the two ends and an aligned phase in the middle. The morphological, mechanical properties and scaffold degradation were investigated. MSCs proliferation, adhesion, morphology and fibroblast markers were evaluated under dynamic culturing. This scaffold were tested if they could induce ligament formation using a rodent model in vivo. Results: Compared with other materials, poly(D,L-lactide-co-glycolide)/poly(ε-caprolactone) (PLGA/PCL) with mass ratio of 1:5 showed appropriate mechanical properties and biodegradability that matched ACLs. After 28 days of dynamic culturing, MSCs were fusiform oriented in the aligned phase and randomly arranged in a paving-stone-like morphology in the random phase. The increased expression of fibroblastic markers demonstrated that only the alignment of nanofibers worked with mechanical stimulation to promote effective fibroblast differentiation. This scaffold was a dense collagenous structure, and there was minimal difference in collagen direction in the orientation phase. Conclusion: Dual-phase electrospun scaffolds had mechanical properties and degradability similar to those of ACLs. They promoted differences in the morphology of MSCs and induced fibroblast differentiation under dynamic culture conditions. Animal experiments showed that ligamentous tissue regenerated well and supported joint stability.

#Electrospun #Biphasic #Anterior cruciate ligament #Tissue engineering

참고문헌 (36)

참고문헌 신청

Nau T / 2002 / A new generation of artificial ligaments in reconstruction of the anterior cruciate ligament. Twoyear follow-up of a randomised trial / J Bone Joint Surg Br 84:356~360

Hamido F / 2011 / The use of the LARS artificial ligament to augment a short or undersized ACL hamstrings tendon graft / Knee 18:373~378

Wei Lee Lim / 2019 / Current Progress in Tendon and Ligament Tissue Engineering / 조직공학과 재생의학 16(6):549~571

Kuo CK / 2010 / Novel strategies in tendon and ligament tissue engineering : advanced biomaterials and regeneration motifs / Sports Med Arthrosc Rehabil Ther Technol 2:20

Bistolfi A / 2021 / Functional results of allograft vs. Autograft tendons in anterior cruciate ligament (ACL) reconstruction at 10-year follow-up / Eur J Orthop Surg Traumatol 31:729~735

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Tang Ya

소속기관 Guizhou University

주요연구분야 공학 > 기타공학 > 의공학

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Tian Jialiang

소속기관 Orthopedic Department Guizhou Provincial People’s Hospital

주요연구분야 공학 > 기타공학 > 의공학

논문수 2 이용수 0

Li Long

소속기관 College of Materials and Metallurgy Guizhou University

주요연구분야 공학 > 기타공학 > 의공학

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Huang Lin

소속기관 College of Materials and Metallurgy Guizhou University

주요연구분야 공학 > 기타공학 > 의공학

논문수 2 이용수 0

Shen Quan

소속기관 College of Materials and Metallurgy Guizhou Unive

주요연구분야 공학 > 기타공학 > 의공학

논문수 2 이용수 0

Guo Shanzhu

소속기관 College of Materials and Metallurgy Guizhou University

주요연구분야 공학 > 기타공학 > 의공학

논문수 2 이용수 0

Jiang Yue

소속기관 College of Materials and Metallurgy Guizhou University

주요연구분야 공학 > 기타공학 > 의공학

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