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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 107 - 114 (8page)
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초록· 키워드
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Implementation of crop improvement programs relies on genetic diversity. To overcome the limited occurrence of natural mutations, researchers and breeders applied diverse methods, ranging from conventional crossing to classical biotechnologies.
Earlier generations of knockout and gain-offunction technologies often result in incomplete gene disruption or random insertions of transgenes into plant genomes. The newly developed editing tool, CRISPR/Cas9 system, not only provides a powerful platform to efficiently modify target traits, but also broadens the scope and prospects of genome editing.
Customized Cas9/guide RNA (gRNA) systems suitable for efficient genomic modification of mammalian cells or plants have been reported. Following successful demonstration of this technology in mammalian cells, CRISPR/Cas9 was successfully adapted in plants, and accumulating evidence of its feasibility has been reported in model plants and major crops.
Recently, a modified version of CRISPR/Cas9 with added novel functions has been developed that enables programmable direct irreversible conversion of a target DNA base. In this review, we summarized the milestone applications of CRISPR/ Cas9 in plants with a focus on major crops. We also present the implications of an improved version of this technology in the current plant breeding programs.
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참고문헌 (54)
참고문헌 신청
Abdallah NA / 2015 / Genome editing for crop improvement: Challenges and opportunities / GM Crops & Food 6:183~205
Altpeter F / 2017 / Genetic Improvement of Sugarcane by Targeted Lossor Gain of Function Mutations using TALEN or CRISPR-Cas9 / Proc. of the Plant and Animal Genome Conference (PAG XXV)
Asano K / 2009 / Isolation and characterization of dominant dwarf mutants, Slr1-d, in rice / Mol. Genet. Genomics 281:223~231
Bibikova M / 2002 / Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases / Genetics 161:1169~1175
Bortesi L / 2015 / The CRISPR/Cas9 system for plant genome editing and beyond / Biotechnology Advances 33:41~52
Altpeter F / 2017 / Genetic Improvement of Sugarcane by Targeted Lossor Gain of Function Mutations using TALEN or CRISPR-Cas9 / Proc. of the Plant and Animal Genome Conference (PAG XXV)
Asano K / 2009 / Isolation and characterization of dominant dwarf mutants, Slr1-d, in rice / Mol. Genet. Genomics 281:223~231
Bibikova M / 2002 / Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases / Genetics 161:1169~1175
Bortesi L / 2015 / The CRISPR/Cas9 system for plant genome editing and beyond / Biotechnology Advances 33:41~52
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