지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한악안면성형재건외과학회 Maxillofacial Plastic Reconstructive Surgery Maxillofacial Plastic Reconstructive Surgery 제40권 제12호
- 발행연도
- 2018.5
- 수록면
- 1 - 6 (6page)
- DOI
- https://doi.org/10.1186/s40902-018-0149-1
이용수
초록· 키워드
오류제보하기
Background: The objective of this study was to evaluate the changes in gene expression after incubation of cells with proteins released from different silk mat layers.
Methods: A silk cocoon from Bombyx mori was separated into four layers of equal thickness. The layers were numbered from 1 to 4 (from the inner to the outer layer). The proteins were released by sonication of a silk mat layer in normal saline. The concentration of proteins was determined by spectrophotometry. They were incubated with RAW264.7 cells, and changes in the expression of genes were evaluated by cDNA microarray analysis and quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).
Results: Layer 1 and 4 groups had higher protein concentrations compared to those in layer 2 and 3 groups. The genes associated with inflammation and angiogenesis showed significantly higher expression in layer 1 and 4 groups. The results of qRT-PCR were in agreement with those of the cDNA microarray analysis.
Conclusions: The silk mat from the middle portion of the silkworm cocoon yielded a lower protein release and caused an insignificant change in the expression of genes that are associated with inflammation and angiogenesis.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Seok H / 2014 / Comparison of silkworm-cocoonderived silk membranes of two different thicknesses for guided bone regeneration / J Craniofac Surg 25:2066~2069
Seong-Gon Kim / 2016 / Comparison of unprocessed silk cocoon and silk cocoon middle layer membranes for guided bone regeneration / Maxillofacial Plastic Reconstructive Surgery 38(2):1~8
권해용 / 2017 / In vivo Bone Regeneration Ability of Different Layers of Natural Silk Cocoon Processed Using an Eco-Friendly Method / Macromolecular Research 25(8):806~816
Zhang Y / 2015 / Comparative proteome analysis of multi-layer cocoon of the silkworm, Bombyx mori / PLoS One 10:e0123403
Mondal M / 2007 / The silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn.—a review / Casp J Envir Sci 5:63~76
Seong-Gon Kim / 2016 / Comparison of unprocessed silk cocoon and silk cocoon middle layer membranes for guided bone regeneration / Maxillofacial Plastic Reconstructive Surgery 38(2):1~8
권해용 / 2017 / In vivo Bone Regeneration Ability of Different Layers of Natural Silk Cocoon Processed Using an Eco-Friendly Method / Macromolecular Research 25(8):806~816
Zhang Y / 2015 / Comparative proteome analysis of multi-layer cocoon of the silkworm, Bombyx mori / PLoS One 10:e0123403
Mondal M / 2007 / The silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn.—a review / Casp J Envir Sci 5:63~76
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Comparison of unprocessed silk cocoon and silk cocoon middle layer membranes for guided bone regeneration
Maxillofacial Plastic Reconstructive Surgery
2016 .02
-
Oral Administration of Silk Peptide Enhances the Maturation and Cytolytic Activity of Natural Killer Cells
Immune Network
2018 .10
-
Protein
대한외과학회 학술대회 초록집
2016 .11
-
Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
생체재료학회지
2015 .06
-
Retrospective comparative clinical study for silk mat application into extraction socket
Maxillofacial Plastic Reconstructive Surgery
2019 .01
-
Antioxidation and anti-inflammatory effects of gamma-irradiated silk sericin and fibroin in H2O2-induced HaCaT Cell
한국실험동물학회 학술발표대회 논문집
2022 .07
-
족삼음경의 락혈에 시술된 침 자극에 의한 NO, NOS, NE 발현 연구
Korean Journal of Acupuncture
2017 .01
-
Quality Control Usage in High-Density Microarrays Reveals Differential Gene Expression Profiles in Ovarian Cancer
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Comparison of Retinal Layer Thicknesses of Highly Myopic Eyes and Normal Eyes
Korean Journal of Ophthalmology
2020 .01
-
Anti-diabetic effect of silk fibroin hydrolysate
한국실험동물학회 학술발표대회 논문집
2017 .08
-
Clinical Outcomes of Silk Patch in Acute Tympanic Membrane Perforation
Clinical and Experimental Otorhinolaryngology
2015 .01
-
Differentially Expressed Proteins in Nitric Oxide-Stimulated NIH/3T3 Fibroblasts: Implications for Inhibiting Cancer Development
Yonsei Medical Journal
2015 .01
-
Microarray Analysis of Gene Expression Alteration in Human Middle Ear Epithelial Cells Induced by Asian Sand Dust
Clinical and Experimental Otorhinolaryngology
2015 .01
-
A Novel Polyclonal Antiserum against Toxoplasma gondii Sodium Hydrogen Exchanger 1
Parasites, Hosts and Diseases
2016 .02
-
Carbon-Fiber Based Microelectrode Array Embedded with a Biodegradable Silk Support for In Vivo Neural Recording
Journal of Korean Medical Science
2019 .01
-
Microarray analysis of anti-diabetic effect of silk fibroin in C57BL/KsJ-db/db mice
한국실험동물학회 학술발표대회 논문집
2015 .08
-
The radioligands with VEGF<sub>121</sub> for angiogenesis of tumor
대한방사성의약품학회지
2018 .01
-
Protein supplements
대한외과학회 학술대회 초록집
2016 .11
-
Silk fibroin hydrolysate inhibits hepatic steatosis and inflammation in liver of type 2 diabetes mice model (C57BL/KsJ-db/db)
한국실험동물학회 학술발표대회 논문집
2017 .08
-
Comparison of Each Retinal Layer Thicknesses between Eyes with Central Retinal Vein Occlusion and Normal Contralateral Eyes
Korean Journal of Ophthalmology
2022 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0