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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 230 - 239 (10page)
- DOI
- 10.9787/KJBS.2021.53.3.230
이용수
초록· 키워드
오류제보하기
Soybean (Glycine max (L.) Merr.) is one of the most important crops with economic value as a source of protein and vegetableoil for human food and animal feed. In recent years, rapidly developed genome editing techniques have shown widespread application prospectsfor gene function studies and for improving important agronomic traits in many crops. Therefore, it is important to establish a highly efficientmethod for protoplast isolation and transient expression systems in soybeans. In this study, we established an efficient method for protoplastisolation and its application to transient gene expression in Korean soybean cultivars. The protoplasts were isolated from leaves, epicotyls,hypocotyls, cotyledons, and etiolated hypocotyls using various combinations of enzyme mixtures. We found that high-quality and large amountsof protoplasts were isolated from the etiolated hypocotyls when incubated for 8 h under conditions of 0.5% cellulase, 0.5% pectinase, and1% viscozyme. In addition, we observed a high transfection efficiency of green fluorescent protein using etiolated hypocotyl protoplasts. Takentogether, our protoplast isolation and transfection method is highly efficient and can be used for gene function and molecular analysis tobetter understand the biological and physiological processes in soybean.
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등록된 정보가 없습니다.
참고문헌 (2)
참고문헌 신청
강문영 / 2020 / A robust genome-editing method for wild plant species Nicotiana attenuata / Plant Biotechnology Reports 14(5):585~598
김혜정 / 2017 / Overexpression of a Chromatin Architecture-Controlling ATPG7 has Positive Effect on Yield Components in Transgenic Soybean / Plant Breeding and Biotechnology 5(3):237~242
김혜정 / 2017 / Overexpression of a Chromatin Architecture-Controlling ATPG7 has Positive Effect on Yield Components in Transgenic Soybean / Plant Breeding and Biotechnology 5(3):237~242
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