Applicability Evaluation of Male-Specific Coliphage-Based Detection Methods for Microbial Contamination Tracking

Kim Gyungcheon; Park Gwoncheol; Kang Seohyun; Lee Sanghee; Park Jiyoung; Ha Jina; Park Kunbawui; Kang Minseok; Cho Min; 신학동

doi:10.4014/jmb.2110.10003

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자료유형: 학술저널

저자정보: Kim Gyungcheon (Department of Food Science and Biotechnology College of Life Science Sejong University Seoul 05006) Park Gwoncheol (Department of Food Science and Biotechnology College of Life Science Sejong University Seoul 05006) Kang Seohyun (Department of Food Science and Biotechnology College of Life Science Sejong University Seoul 05006) Lee Sanghee (Department of Food Science and Biotechnology College of Life Science Sejong University Seoul 05006) Park Jiyoung (Department of Food Science and Biotechnology College of Life Science Sejong University Seoul 05006) Ha Jina (Department of Food Science and Biotechnology College of Life Science Sejong University Seoul 05006) Park Kunbawui (Food Safety and Processing Research Division National Institute of Fisheries Science Busan 46083 Re) Kang Minseok (Division of Biotechnology SELS Center College of Environmental and Bioresource Sciences Jeonbuk Nat) Cho Min (Division of Biotechnology SELS Center College of Environmental and Bioresource Sciences Jeonbuk Nat) 신학동 (세종대학교)

저널정보: 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제12호

발행연도: 2021.12

수록면: 1,709 - 1,715 (7page)

DOI: 10.4014/jmb.2110.10003

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Outbreaks of food poisoning due to the consumption of norovirus-contaminated shellfish continue to occur. Male-specific (F+) coliphage has been suggested as an indicator of viral species due to the association with animal and human wastes. Here, we compared two methods, the double agar overlay and the quantitative real-time PCR (RT-PCR)-based method, for evaluating the applicability of F+ coliphage-based detection technique in microbial contamination tracking of shellfish samples. The RT-PCR-based method showed 1.6-39 times higher coliphage PFU values from spiked shellfish samples, in relation to the double agar overlay method. These differences indicated that the RT-PCRbased technique can detect both intact viruses and non-particle-protected viral DNA/RNA, suggesting that the RT-PCR based method could be a more efficient tool for tracking microbial contamination in shellfish. However, the virome information on F+ coliphage-contaminated oyster samples revealed that the high specificity of the RT-PCR- based method has a limitation in microbial contamination tracking due to the genomic diversity of F+ coliphages. Further research on the development of appropriate primer sets for microbial contamination tracking is therefore necessary. This study provides preliminary insight that should be examined in the search for suitable microbial contamination tracking methods to control the sanitation of shellfish and related seawater.

#Male-specific coliphage #double agar overlay method #RT-PCR #virome

참고문헌 (42)

참고문헌 신청

Le Guyader FS / 2012 / Transmission of viruses through shellfish : when specific ligands come into play / Curr. Opin. Virol 2:103~110

Lopman BA / 2016 / The vast and varied global burden of norovirus : Prospects for prevention and control / PLoS Med 13:e1001999

Ministry of Food and Drug Safety (South Korea) / 2021 / Ministry of Food and Drug Safety White Paper 2021

Liang Z / 2013 / Impact of mixed land-use practices on the microbial water quality in a subtropical coastal watershed / Sci. Total Environ 449:426~433

Ueki Y / 2007 / Persistence of caliciviruses in artificially contaminated oysters during depuration / Appl. Environ. Microbiol 73:5698~5701

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이 논문의 저자 정보

Kim Gyungcheon

소속기관 Department of Food Science and Biotechnology and Carbohydrate Bioproduct Research Center College of Life Science Sejong University Seoul 05006 Republic of Korea

주요연구분야 자연과학 > 생물학

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Park Gwoncheol