지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제12호
- 발행연도
- 2021.12
- 수록면
- 1,624 - 1,631 (8page)
- DOI
- 10.4014/jmb.2109.09039
이용수
초록· 키워드
오류제보하기
Prodigiosin as a high-valued compound, which is a microbial secondary metabolite, has the potential for antioxidant and anticancer effects. However, the large-scale production of functionally active Hahella chejuensis-derived prodigiosin by fermentation in a cost-effective manner has yet to be achieved. In the present study, we established carbon source-optimized medium conditions, as well as a procedure for producing prodigiosin by fermentation by culturing H. chejuensis using 10 L and 200 L bioreactors. Our results showed that prodigiosin productivity using 250 ml flasks was higher in the presence of glucose than other carbon sources, including mannose, sucrose, galactose, and fructose, and could be scaled up to 10 L and 200 L batches. Productivity in the glucose (2.5 g/l) culture while maintaining the medium at pH 6.89 during 10 days of cultivation in the 200 L bioreactor was measured and increased more than productivity in the basal culture medium in the absence of glucose. Prodigiosin production from 10 L and 200 L fermentation cultures of H. chejuensis was confirmed by high-performance liquid chromatography (HPLC) and liquid chromatography?mass spectrometry (LC?MS) analyses for more accurate identification. Finally, the anticancer activity of crude extracted prodigiosin against human cancerous leukemia THP-1 cells was evaluated and confirmed at various concentrations. Conclusively, we demonstrate that culture conditions for H. chejuensis using a bioreactor with various parameters and ethanol-based extraction procedures were optimized to mass-produce the marine bacterium-derived high purity prodigiosin associated with anti-cancer activity.
목차
등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Wang SL / 2020 / Production and potential applications of bioconversion of chitin and protein-containing fishery byproducts into prodigiosin : a review / Molecules 25:2744
김성진 / 2008 / Statistical Optimization of Medium Components for the Production of Prodigiosin by Hahella chejuensis KCTC 2396 / Journal of Microbiology and Biotechnology 18(12):1903~1907
Williamson NR / 2005 / Biosynthesis of the red antibiotic, prodigiosin, in Serratia : identification of a novel 2-methyl-3-n-amyl-pyrrole(MAP)assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces / Mol. Microbiol 56:971~989
Harris AKP / 2004 / The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species-and strain-dependent genome context variation / Microbiology(Reading) 150:3547~3560
Darshan N / 2015 / Prodigiosin and its potential applications / J. Food Sci. Technol 52:5393~5407
김성진 / 2008 / Statistical Optimization of Medium Components for the Production of Prodigiosin by Hahella chejuensis KCTC 2396 / Journal of Microbiology and Biotechnology 18(12):1903~1907
Williamson NR / 2005 / Biosynthesis of the red antibiotic, prodigiosin, in Serratia : identification of a novel 2-methyl-3-n-amyl-pyrrole(MAP)assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces / Mol. Microbiol 56:971~989
Harris AKP / 2004 / The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species-and strain-dependent genome context variation / Microbiology(Reading) 150:3547~3560
Darshan N / 2015 / Prodigiosin and its potential applications / J. Food Sci. Technol 52:5393~5407
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effects of Hahella chejuensis -Derived Prodigiosin on UV-Induced ROS Production, Inflammation and Cytotoxicity in HaCaT Human Skin Keratinocytes
Journal of Microbiology and Biotechnology
2021 .01
-
Antimicrobial Activity of Prodigiosin from Hahella chejuensis against Cutibacterium acnes
한국미생물학회 학술대회논문집
2022 .10
-
Antimicrobial Activity of Prodigiosin from Serratia sp. PDGS120915 Against Intestinal Pathogenic Bacteria
한국미생물·생명공학회지
2019 .01
-
Anti-MRSA Properties of Prodigiosin from Serratia sp. PDGS 120915
생명과학회지
2015 .01
-
Elicitation of the Hypersensitive Response-like Cell Death on Nicotiana benthamiana by a Marine Bacterium Hahella chejuensis
한국미생물학회 학술대회논문집
2017 .04
-
A Marine Bacterium with Animal-Pathogen-Like Type III Secretion Elicits the Nonhost Hypersensitive Response in a Land Plant
The Plant Pathology Journal
2023 .12
-
Hahella aquimaris sp. nov., Isolated from Aquaculture Seawater
한국미생물학회 학술대회논문집
2022 .10
-
Increased Production of Ginsenoside Compound K by Optimizing the Feeding of American Ginseng Extract during Fermentation by Aspergillus tubingensis
Journal of Microbiology and Biotechnology
2022 .07
-
Saccharomycopsis fibuligera로 발효된 황매화 추출물의항산화 및 항염효과
대한임상검사과학회지
2022 .09
-
Optimization of a Fermentation Process to Improve Microbial Production of Heme
한국미생물학회 학술대회논문집
2022 .10
-
Bioethanol Production from Sugarcane Molasses by Fed-Batch Fermentation Systems Using Instant Dry Yeast
한국미생물·생명공학회지
2023 .06
-
Development of a High-Titer Culture Medium for the Production of Cholesterol by Engineered Saccharomyces cerevisiae and Its Fed-Batch Cultivation Strategy
Journal of Microbiology and Biotechnology
2022 .09
-
히알루론산 생산성 향상을 위한 Streptococcus zooepidemicus 균주 개량 및 발효조 배양공정 최적화
한국미생물·생명공학회지
2020 .01
-
Optimization of Medium Composition for Biomass Production of Lactobacillus plantarum 200655 Using Response Surface Methodology
Journal of Microbiology and Biotechnology
2021 .01
-
Starter Cultures for Kimchi Fermentation
한국미생물학회 학술대회논문집
2015 .04
-
Microbiota and Physicochemical Analysis on Traditional Kocho Fermentation Enhancer to Reduce Losses (Gammaa) in the Highlands of Ethiopia
한국미생물·생명공학회지
2018 .01
-
Study on Optimization of Liquid Fermentation Medium and Antitumor Activity of the Mycelium on Phyllopora lonicerae
Journal of Microbiology and Biotechnology
2024 .09
-
Feasibility Study on Long-Term Continuous Ethanol Production from Cassava Supernatant by Immobilized Yeast Cells in Packed Bed Reactor
Journal of Microbiology and Biotechnology
2020 .01
-
LAB Fermentation Improves Production of Bioactive Compounds and Antioxidant Activity of Withania somnifera Extract and Its Metabolic Signatures as Revealed by LC-MS/MS
Journal of Microbiology and Biotechnology
2022 .04
-
Comparison of Liquid and Solid-State Fermentation Processes for the Production of Enzymes and Beta-Glucan from Hulled Barley
Journal of Microbiology and Biotechnology
2022 .03
이 논문의 저자 정보
최근 본 자료
댓글(0)
0