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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제9호
- 발행연도
- 2021.9
- 수록면
- 1,305 - 1,310 (6page)
- DOI
- 10.4014/jmb.2106.06009
이용수
초록· 키워드
오류제보하기
Shikimate is a key high-demand metabolite for synthesizing valuable antiviral drugs, such as the anti-influenza drug, oseltamivir (Tamiflu). Microbial-based strategies for shikimate production have been developed to overcome the unstable and expensive supply of shikimate derived from traditional plant extraction processes. In this study, a microbial cell factory using Corynebacterium glutamicum was designed to overproduce shikimate in a fed-batch culture system. First, the shikimate kinase gene (aroK) responsible for converting shikimate to the next step was disrupted to facilitate the accumulation of shikimate. Several genes encoding the shikimate bypass route, such as dehydroshikimate dehydratase (QsuB), pyruvate kinase (Pyk1), and quinate/shikimate dehydrogenase (QsuD), were disrupted sequentially. An artificial operon containing several shikimate pathway genes, including aroE, aroB, aroF, and aroG were overexpressed to maximize the glucose uptake and intermediate flux. The rationally designed shikimate-overproducing C. glutamicum strain grown in an optimized medium produced approximately 37.3 g/l of shikimate in 7-L fed-batch fermentation. Overall, rational cell factory design and culture process optimization for the microbial-based production of shikimate will play a key role in complementing traditional plant-derived shikimate production processes.
목차
등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Candeias NR / 2018 / Production and synthetic modification of shikimic acid / Chem. Rev 118:10458~10550
Kogure T / 2016 / Metabolic engineering of Corynebacterium glutamicum for shikimate overproduction by growth-arrested cell reaction / Metab. Eng 38:204~216
Bochkov DV / 2012 / Shikimic acid : review of its analytical, isolation, and purification techniques from plant and microbial sources / J. Chem. Biol 5:5~17
Ghosh S / 2012 / Production of shikimic acid / Biotechnol. Adv 30:1425~1431
Krämer M / 2003 / Metabolic engineering for microbial production of shikimic acid / Metab. Eng 5:277~283
Kogure T / 2016 / Metabolic engineering of Corynebacterium glutamicum for shikimate overproduction by growth-arrested cell reaction / Metab. Eng 38:204~216
Bochkov DV / 2012 / Shikimic acid : review of its analytical, isolation, and purification techniques from plant and microbial sources / J. Chem. Biol 5:5~17
Ghosh S / 2012 / Production of shikimic acid / Biotechnol. Adv 30:1425~1431
Krämer M / 2003 / Metabolic engineering for microbial production of shikimic acid / Metab. Eng 5:277~283
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