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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제8호
- 발행연도
- 2018.1
- 수록면
- 1,384 - 1,390 (7page)
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초록· 키워드
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Glyphosate inhibits the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway. A mutant of EPSPS from Pantoea sp. was identified using sitedirected mutagenesis. The mutant showed significantly improved glyphosate resistance. The mutant had mutations in three amino acids: Gly97 to Ala, Thr 98 to Ile, and Pro 102 to Ser. These mutation sites in Escherichia coli have been studied as significant active sites of glyphosate resistance. However, in our research, they were found to jointly contribute to the improvement of glyphosate tolerance. In addition, the level of glyphosate tolerance in transgenic Arabidopsis confirmed the potentiality of the mutant in breeding glyphosateresistant plants.
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참고문헌 (31)
참고문헌 신청
Zobiole L / 2012 / Glyphosate effects on photosynthesis, nutrient accumulation, and nodulation in glyphosate-resistant soybean / J. Plant Nutr. Soil Sci. 175:319~330
Baerson SR / 2002 / Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase / Plant Physiol. 129:1265~1275
Yi SY / 2016 / A novel naturally occurring class I 5-enolpyruvylshikimate-3-phosphate synthase from Janibacter sp. confers high glyphosate tolerance to rice / Sci. Rep. 6:19104
Cao G / 2012 / A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants / PLoS One 7:e38718
Priestman MA / 2005 / 5-Enolpyruvylshikimate-3-phosphate synthase from Staphylococcus aureus is insensitive to glyphosate / FEBS Lett. 579:728~732
Baerson SR / 2002 / Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase / Plant Physiol. 129:1265~1275
Yi SY / 2016 / A novel naturally occurring class I 5-enolpyruvylshikimate-3-phosphate synthase from Janibacter sp. confers high glyphosate tolerance to rice / Sci. Rep. 6:19104
Cao G / 2012 / A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants / PLoS One 7:e38718
Priestman MA / 2005 / 5-Enolpyruvylshikimate-3-phosphate synthase from Staphylococcus aureus is insensitive to glyphosate / FEBS Lett. 579:728~732
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