지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.9
- 수록면
- 305 - 315 (11page)
- DOI
- 10.48022/mbl.2104.04002
이용수
초록· 키워드
오류제보하기
The cotton leafworm, Spodoptera littoralis, is a major pest in Egypt and many countries worldwide, and causes heavy economic losses. As a result, management measures to control the spread of the worm are required. S. littoralis nucleopolyhedrovirus (SpliNPV) is one of the most promising bioagents for the efficient control of insect pests. In this study, a chitinase gene (chitA) of a 1.8 kb DNA fragment was cloned and fully characterized from SpliNPV-EG1, an Egyptian isolate. A sequence of 601 amino acids was deduced when the gene was completely sequenced with a predicted molecular mass of 67 kDa for the preprotein. Transcriptional analyses using reverse transcription polymerase chain reaction (RT-PCR) revealed that chitA transcripts were detected first at 12 h post infection (hpi) and remained detectable until 168 hpi, suggesting their transcriptional regulation from a putative late promoter motif. In addition, quantitative analysis using quantitative RT-PCR showed a steady increase of 7.86-fold at 12 hpi in chitA transcription levels, which increased up to 71.4-fold at 120 hpi. An approximately 50 kDa protein fragment with chitinolytic activity was purified from ChitA-induced bacterial culture and detected by western blotting with an antirecombinant SpliNPV chitinase antibody. Moreover, purification of the expressed ChitA recombinant protein showed in vitro growth inhibition of two different fungi species, Fusarium solani and F. oxysporum, confirming that the enzyme assembly and activity was correct. The results supported the potential role and application of the SpliNPV-ChitA protein as a synergistic agent in agricultural fungal and pest control programs.
목차
등록된 정보가 없습니다.
참고문헌 (48)
참고문헌 신청
Kumar B / 2016 / In:Ecofriendly pest management for food security / Academic Press :25~61
van Oers MM / 2015 / Thirty years of baculovirusinsect cell protein expression: From dark horse to mainstream technology / J. Gen. Virol 96:6~23
Elgaied L / 2017 / Expression of tomato yellow leaf curl virus coat protein using baculovirus expression system and evaluation of its utility as a viral antigen / 3 Biotech 7:269
Elmenofy W / 2020 / Expression of 1B capsid protein of Foot-and-mouth disease virus (FMDV) using baculovirus expression system and its validation in detecting SAT 2- specific antisera / Peer J. 8:e8946
Theilmann DA / 2005 / “Baculoviridae” Eighth report of the international committee on taxonomy of viruses / Elsevier Academic Press :177~185
van Oers MM / 2015 / Thirty years of baculovirusinsect cell protein expression: From dark horse to mainstream technology / J. Gen. Virol 96:6~23
Elgaied L / 2017 / Expression of tomato yellow leaf curl virus coat protein using baculovirus expression system and evaluation of its utility as a viral antigen / 3 Biotech 7:269
Elmenofy W / 2020 / Expression of 1B capsid protein of Foot-and-mouth disease virus (FMDV) using baculovirus expression system and its validation in detecting SAT 2- specific antisera / Peer J. 8:e8946
Theilmann DA / 2005 / “Baculoviridae” Eighth report of the international committee on taxonomy of viruses / Elsevier Academic Press :177~185
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