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논문 기본 정보

자료유형
학술저널
저자정보
Ham, Youngseok (Department of Forest Products and Biotechnology, Kookmin University) Park, Han-Saem (Department of Forest Products and Biotechnology, Kookmin University) Kim, Yeong-Suk (Department of Forest Products and Biotechnology, Kookmin University) Kim, Tae-Jong (Department of Forest Products and Biotechnology, Kookmin University)
저널정보
한국목재공학회 목재공학(Journal of the Korean Wood Science and Technology) 목재공학 제47권 제3호
발행연도
2019.1
수록면
371 - 380 (10page)

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Termites are pests that cause serious economic and cultural damage by digesting wood cellulose. Termites are arthropods and have an epidermis surrounded by a chitin layer. To maintain a healthy epidermis, termites have chitinase (${\beta}$-1,4-poly-N-acetyl glucosamidinase, EC 3.2.1.14), an enzyme that hydrolyzes the ${\beta}$-1,4 bond of chitin. In this study, the amino acid sequence of the gene, which is presumed to be termite chitinolytic enzyme (NCBI accession no. KC477099), was obtained from a transcriptomic analysis of Reticulitermes speratus KMT001 in Bukhan Mountain, Korea. An NCBI protein BLAST search confirmed that the protein is a glycoside hydrolase family 18 (GH18). The highest homology value found was 47%, with a chitinase from Araneus ventricosus. Phylogenetic analysis indicated that the KC477099 protein has the same origins as those of arthropods but has a very low similarity with other arthropod chitinases, resulting in separation at an early stage of evolution. The KC477099 protein contains two conserved motifs, which encode the general enzymatic characteristics of the GH18 group. The amino acid sequences $Asp^{156}-Trp^{157}-Glu^{158}$, which play an important role in the enzymatic activity of the GH18 group, were also present. This study suggests that the termite KC477099 protein is a new type of chitinase, which is evolutionarily distant from other insect chitinases.

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