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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.12
- 수록면
- 911 - 919 (9page)
- DOI
- 10.14348/molcells.2021.0237
이용수
초록· 키워드
오류제보하기
The virus-induced genome editing (VIGE) system aims to induce targeted mutations in seeds without requiring any tissue culture. Here, we show that tobacco rattle virus (TRV) harboring guide RNA (gRNA) edits germ cells in a wild tobacco, Nicotiana attenuata, that expresses Streptococcus pyogenes Cas9 (SpCas9). We first generated N. attenuata transgenic plants expressing SpCas9 under the control of 35S promoter and infected rosette leaves with TRV carrying gRNA. Gene-edited seeds were not found in the progeny of the infected N. attenuata. Next, the N. attenuata ribosomal protein S5 A (RPS5A) promoter fused to SpCas9 was employed to induce the heritable gene editing with TRV. The RPS5A promoter-driven SpCas9 successfully produced monoallelic mutations at three target genes in N. attenuata seeds with TRV-delivered guide RNA. These monoallelic mutations were found in 2%-6% seeds among M1 progenies. This editing method provides an alternative way to increase the heritable editing efficacy of VIGE.
목차
등록된 정보가 없습니다.
참고문헌 (40)
참고문헌 신청
안율균 / 2013 / Development of an Efficient Agrobacterium-Mediated Transformation System and Production of Herbicide-Resistant Transgenic Plants in Garlic (Allium sativum L.) / Molecules and Cells 36(2):158~162
Ali, Z. / 2015 / Efficient virus-mediated genome editing in plants using the CRISPR/Cas9 system / Mol. Plant 8:1288~1291
Ali, Z. / 2018 / Pea early-browning virusmediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis / Virus Res 244:333~337
Alonso, J.M. / 2006 / Moving forward in reverse: genetic technologies to enable genome-wide phenomic screens in Arabidopsis / Nat. Rev. Genet 7:524~536
Altpeter, F. / 2016 / Advancing crop transformation in the era of genome editing / Plant Cell 28:1510~1520
Ali, Z. / 2015 / Efficient virus-mediated genome editing in plants using the CRISPR/Cas9 system / Mol. Plant 8:1288~1291
Ali, Z. / 2018 / Pea early-browning virusmediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis / Virus Res 244:333~337
Alonso, J.M. / 2006 / Moving forward in reverse: genetic technologies to enable genome-wide phenomic screens in Arabidopsis / Nat. Rev. Genet 7:524~536
Altpeter, F. / 2016 / Advancing crop transformation in the era of genome editing / Plant Cell 28:1510~1520
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