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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.7
- 수록면
- 631 - 638 (8page)
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Spermatogonial stem cells (SSCs) derived from mouse testis are unipotent in regard of spermatogenesis. Our previous study demonstrated that SSCs can be fully reprogrammed into plu-ripotent stem cells, so called germline-derived pluripotent stem cells (gPS cells), on feeder cells (mouse embryonic fibroblasts), which supports SSC proliferation and induction of pluripotency. Because of an uncontrollable microenvironment caused by interactions with feeder cells, feeder-based SSC reprogramming is not suitable for elucidation of the self-reprogramming mechanism by which SSCs are converted into pluripotent stem cells. Recently, we have established a Matrigel-based SSC expansion culture system that allows long-term SSC prolifera-tion without mouse embryonic fibroblast support. In this study, we developed a new feeder-free SSC self-reprogramming protocol based on the Matrigel-based culture system. The gPS cells generated using a feeder-free reprogramming system showed pluripotency at the molecular and cellular levels. The differentiation potential of gPS cells was confirmed in vitro and in vivo. Our study shows for the first time that the induction of SSC pluripotency can be achieved without feeder cells. The newly developed feeder-free self-reprogramming system could be a useful tool to reveal the mechanism by which unipotent cells are self reprogrammed into pluripotent stem cells.
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참고문헌 (37)
참고문헌 신청
Brons, I.G. / 2007 / Derivation of pluripotent epiblast stem cells from mammalian embryos / Nature 448:191~195
Brustle, O. / 1999 / Embryonic stem cell-derived glial precursors: a source of myelinating transplants / Science 285:754~756
최나영 / 2014 / A Novel Feeder-Free Culture System for Expansion of Mouse Spermatogonial Stem Cells / Molecules and Cells 37(6):473~479
Dann, C.T. / 2008 / Spermatogonial stem cell self-renewal requires OCT4, a factor downregulated during retinoic acid-induced differentiation / Stem Cells 26:2928~2937
Diaferia, G.R. / 2011 / Systematic chromosomal analysis of cultured mouse neural stem cell lines / Stem Cells Dev 20:1411~1423
Brustle, O. / 1999 / Embryonic stem cell-derived glial precursors: a source of myelinating transplants / Science 285:754~756
최나영 / 2014 / A Novel Feeder-Free Culture System for Expansion of Mouse Spermatogonial Stem Cells / Molecules and Cells 37(6):473~479
Dann, C.T. / 2008 / Spermatogonial stem cell self-renewal requires OCT4, a factor downregulated during retinoic acid-induced differentiation / Stem Cells 26:2928~2937
Diaferia, G.R. / 2011 / Systematic chromosomal analysis of cultured mouse neural stem cell lines / Stem Cells Dev 20:1411~1423
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