지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.6
- 수록면
- 473 - 479 (7page)
- DOI
- 10.14348/molcells.2014.0080
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초록· 키워드
오류제보하기
Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation of feeder cells is needed at each subculture. In this study, we developed a Matrigel-based feeder-free culture system for long-term propagation of SSCs. Although several in vitro SSC culture systems without feeder cells have been previously described, our Matrigel-based feeder-free culture system is time- and cost- effective, and preserves self-renewability of SSCs. In addition, the growth rate of SSCs cultured using our newly developed system is equivalent to that in feeder cultures. We confirmed that the feeder-free cultured SSCs expressed germ cell markers both at the mRNA and protein levels. Furthermore, the functionality of feeder-free cultured SSCs was confirmed by their transplantation into germ cell-depleted mice. These results suggest that our newly developed feeder-free culture system provides a simple approach to maintaining SSCs in vitro and studying the basic biology of SSCs, including determination of their fate.
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참고문헌 (27)
참고문헌 신청
Bodnar, M. S. / 2004 / Propagation and maintenance of undifferentiated human embryonic stem cells / Stem Cells Dev 13:243~253
Braam, S. R. / 2008 / Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin / Stem Cells 26:2257~2265
Brinster, R. L. / 1994 / Spermatogenesis following male germ-cell transplantation / Proc. Natl. Acad. Sci. USA 91:11298~11302
Kanatsu-Shinohara, M. / 2003 / Long-term proliferation in culture and germline transmission of mouse male germline stem cells / Biol. Reprod 69:612~616
Kanatsu-Shinohara, M. / 2005 / Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions / Biol. Reprod 72:985~991
Braam, S. R. / 2008 / Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin / Stem Cells 26:2257~2265
Brinster, R. L. / 1994 / Spermatogenesis following male germ-cell transplantation / Proc. Natl. Acad. Sci. USA 91:11298~11302
Kanatsu-Shinohara, M. / 2003 / Long-term proliferation in culture and germline transmission of mouse male germline stem cells / Biol. Reprod 69:612~616
Kanatsu-Shinohara, M. / 2005 / Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions / Biol. Reprod 72:985~991
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