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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.11
- 수록면
- 828 - 836 (9page)
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초록· 키워드
오류제보하기
Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in mon-ocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our gen-erated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the subcellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.
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참고문헌 (41)
참고문헌 신청
Chalfie, M. / 1994 / Green fluorescent protein as a marker for gene expression / Science 263:802~805
Collings, D. A. / 2013 / Subcellular localization of transiently expressed fluorescent fusion proteins / Methods Mol. Biol 1069:227~258
Cutler, S. R. / 2000 / Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency / Proc. Natl. Acad. Sci. USA 97:3718~3723
Day, R. N. / 2009 / The fluorescent protein palette: tools for cellular imaging / Chem. Soc. Rev 38:2887~2921
Detmer, S. A. / 2007 / Functions and dysfunctions of mitochondrial dynamics / Nat. Rev. Mol. Cell Biol 8:870~900
Collings, D. A. / 2013 / Subcellular localization of transiently expressed fluorescent fusion proteins / Methods Mol. Biol 1069:227~258
Cutler, S. R. / 2000 / Random GFP::cDNA fusions enable visualization of subcellular structures in cells of Arabidopsis at a high frequency / Proc. Natl. Acad. Sci. USA 97:3718~3723
Day, R. N. / 2009 / The fluorescent protein palette: tools for cellular imaging / Chem. Soc. Rev 38:2887~2921
Detmer, S. A. / 2007 / Functions and dysfunctions of mitochondrial dynamics / Nat. Rev. Mol. Cell Biol 8:870~900
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