개인구독
소속 기관이 없으신 경우, 개인 정기구독을 하시면 저렴하게
논문을 무제한 열람 이용할 수 있어요.
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
DNA metabarcoding is currently used for large-scale taxonomic identification to understand the community composition in various marine ecosystems. However, before being widely used in this emerging field, this experimental and analytic approach still has several technical challenges to overcome, such as polymerase chain reaction (PCR) bias, and lack of well-established metabarcoding markers, a task which is difficult but not impossible to achieve. In this study, we present an adapted PCR-free small-organelles enriched metagenomics (SoEM) method for marine biodiversity assessment. To avoid PCR bias and random artefacts, we extracted target DNA sequences without PCR amplification from marine environmental samples enriched with small organelles including mitochondria and plastids because their genome sequences provide a valuable source of molecular markers for phylogenetic analysis. To experimentally enrich small organelles, we performed subcellular fractionation using modified differential centrifugation for marine environmental DNA samples. To validate our SoEM method, two marine environmental samples from the coastal waters were tested the taxonomic capturing capacity against that of traditional DNA metabarcoding method. Results showed that, regardless of taxonomic levels, at least 3-fold greater numbers of taxa were identified in our SoEM method, compared to those identified by the conventional multi-locus DNA metabarcoding method. The SoEM method is thus effective and accurate for identifying taxonomic diversity and presents a useful alternative approach for evaluating biodiversity in the marine environment.
목차
등록된 정보가 없습니다.
참고문헌 (64)
참고문헌 신청
Andújar, C. / 2015 / Phylogenetic community ecology of soil biodiversity using mitochondrial metagenomics / Mol. Ecol 24:3603~3617
Andújar, C. / 2018 / Why the COI barcode should be the community DNA metabarcode for the metazoa / Mol. Ecol 27:3968~3975
Ansorge, W. J. / 2009 / Next-generation DNA sequencing techniques / N. Biotechnol 25:195~203
Appeltans, W. / 2012 / The magnitude of global marine species diversity / Curr. Biol 22:2819~2202
Bellemain, E. / 2010 / ITS as an environmental DNA barcode for fungi : an in silico approach reveals potential PCR biases / BMC Microbiol 10:189
Andújar, C. / 2018 / Why the COI barcode should be the community DNA metabarcode for the metazoa / Mol. Ecol 27:3968~3975
Ansorge, W. J. / 2009 / Next-generation DNA sequencing techniques / N. Biotechnol 25:195~203
Appeltans, W. / 2012 / The magnitude of global marine species diversity / Curr. Biol 22:2819~2202
Bellemain, E. / 2010 / ITS as an environmental DNA barcode for fungi : an in silico approach reveals potential PCR biases / BMC Microbiol 10:189
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Importance of taxonomic research for biodiversity of Korea
Journal of species research
2016 .01
-
Cytogenetic Analysis of Bienertia sinuspersici Akhani as the First Step in Genome Sequencing
Genes & Genomics
2020 .01
-
대기입자상물질시료의곰팡이메타게놈분석을위한 DNA추출및 PCR조건최적화
한국미생물·생명공학회지
2023 .03
-
RNA outperforms DNA-based metabarcoding in assessing the diversity and response of microeukaryotes to environmental variables in the Arctic Ocean
극지연구소 초록집
2024 .07
-
Metagenomics of Light Harvesting in the Marine Environment
한국미생물학회 학술대회논문집
2016 .04
-
Visualization of Multicolored in vivo Organelle Markers for Co-Localization Studies in Oryza sativa
Molecules and Cells
2017 .11
-
Efficacy of reduced-size short tandem repeat PCR analysis for degraded DNA samples
Genes & Genomics
2021 .01
-
Optimized Metavirome Analysis of Marine DNA Virus Communities for Taxonomic Profiling
극지연구소 초록집
2023 .07
-
Metagenomic Analysis Exploring Taxonomic and Functional Diversity in Dermatologic Surgical Instruments
한국미생물학회 학술대회논문집
2019 .04
-
DNA-based information storage
한국진공학회 학술발표회초록집
2017 .02
-
Application study of PCR additives to improve the split peaks in direct PCR
분석과학
2019 .08
-
Structure and function of Zalpha, a Z conformation-specific nucleic acid binding domain
Biodesign
2015 .12
-
출토 옛사람 뼈와 치아의 DNA 분해지수 및 정량 비교 연구
한국분석과학회 학술대회
2021 .11
-
Application of Dual Metabarcoding Platforms for the Meso- and Macrozooplankton Taxa in the Ross Sea
극지연구소 초록집
2023 .07
-
A novice’s guide to analyzing NGS-derived organelle and metagenome data
Algae
2016 .01
-
Covariance of Marine Nucleocytoplasmic Large DNA Viruses witih Eukaryotic Plankton Communities in the Sub-Arctic Kongsfjorden Ecosystem: A Metagenomic Analysis of Marine Microbial Ecosystems
극지연구소 초록집
2024 .07
-
Bacterial Epi-metagenomics : Measuring DNA Methylation Patterns in Bacterial Community Level
한국미생물학회 학술대회논문집
2016 .04
-
[INTERVIEW] THE DNA DETECTIVE DNA 탐정
BBC 사이언스
2022 .06
-
세포 속 액체상 소기관
물리학과 첨단기술
2025 .02
-
Highly Sensitive Detection of Low-Abundance White Spot Syndrome Virus by a Pre-Amplification PCR Method
Journal of Microbiology and Biotechnology
2017 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0