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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.5
- 수록면
- 339 - 345 (7page)
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초록· 키워드
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Retroviral and lentiviral vectors are mostly pseudotyped and often purified and concentrated via ultracentrifugation. In this study, we quantified and compared the stabilities of retroviral [murine leukemia virus (MLV)-based] and lentiviral [human immunodeficiency virus (HIV)-1-based] vectors pseudotyped with relatively mechanically stable envelope proteins, vesicular stomatitis virus glycoproteins (VSVGs), and the influenza virus WSN strain envelope proteins against ultracentrifugation. Lentiviral genomic and functional particles were more stable than the corresponding retroviral particles against ultracen-trifugation when pseudotyped with VSVGs. However, both retroviral and lentiviral particles were unstable when pseudotyped with the influenza virus WSN strain envelope proteins. Therefore, the stabilities of pseudotyped retroviral and lentiviral vectors against ultracentrifugation process are a function of not only the type of envelope proteins, but also the type of viral internal core (MLV or HIV-1 core). In addition, the fraction of functional viral particles among genomic viral particles greatly varied at times during packaging, depending on the type of envelope proteins used for pseudotyping and the viral internal core.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Akpinar, F. / 2015 / Characterization of vesicular stomatitis virus populations by tunable resistive pulse sensing / J. Virol. Methods 218:71~76
Beer, C. / 2003 / The temperature stability of mouse retroviruses depends on the cholesterol levels of viral lipid shell and cellular plasma membrane / Virology 308:137~146
Chai, N. / 2007 / Assembly of hepatitis B virus envelope proteins onto a lentivirus pseudotype that infects primary human hepatocytes / J. Virol 81:10897~10904
Cronin, J. / 2005 / Altering the tropism of lentiviral vectors through pseudotyping / Curr. Gene Ther 5:387~398
Galipeau, J. / 1999 / Vesicular stomatitis virus G pseudotyped retrovector mediates effective in vivo suicide gene delivery in experimental brain cancer / Cancer Res 59:2384~2394
Beer, C. / 2003 / The temperature stability of mouse retroviruses depends on the cholesterol levels of viral lipid shell and cellular plasma membrane / Virology 308:137~146
Chai, N. / 2007 / Assembly of hepatitis B virus envelope proteins onto a lentivirus pseudotype that infects primary human hepatocytes / J. Virol 81:10897~10904
Cronin, J. / 2005 / Altering the tropism of lentiviral vectors through pseudotyping / Curr. Gene Ther 5:387~398
Galipeau, J. / 1999 / Vesicular stomatitis virus G pseudotyped retrovector mediates effective in vivo suicide gene delivery in experimental brain cancer / Cancer Res 59:2384~2394
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