IFIT1 Expression Patterns Induced by H9N2 Virus and Inactivated Viral Particle in Human Umbilical Vein Endothelial Cells and Bronchus Epithelial Cells

Bo Feng; Qian Zhang; Jianfang Wang; Hong Dong; Xiang Mu; Ge Hu; Tao Zhang

doi:10.14348/molcells.2018.2091

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자료유형: 학술저널

저자정보: Bo Feng (Beijing University of Agriculture) Qian Zhang (Beijing University of Agriculture) Jianfang Wang (Beijing University of Agriculture) Hong Dong (Beijing University of Agriculture) Xiang Mu (Beijing University of Agriculture) Ge Hu (Beijing University of Agriculture) Tao Zhang (Beijing University of Agriculture)

저널정보: 한국분자세포생물학회 Molecules and Cells Molecules and Cells 제41권 제4호

발행연도: 2018.3

수록면: 271 - 281 (11page)

DOI: 10.14348/molcells.2018.2091

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IFIT1 (also known as ISG56) is a member of the interferoninducible protein with tetratricopeptide repeats (IFITs) family. IFITs are strongly induced by type I interferon (IFN), doublestranded RNA and virus infection. Here, we investigated IFIT1 expression in human umbilical vein endothelial cells (HUVECs) and in human bronchus epithelial cells (BEAS-2Bs) induced by the H9N2 virus and inactivated viral particle at different time points. We also investigated the effect of H9N2 virus and viral particle infection on IFN-α/β production, and assessed whether hemagglutinin or neuraminidase protein induced IFIT1 expression. Results showed that both H9N2 virus infection and viral particle inoculation induced the expression of IFIT1 at mRNA and protein levels in the two cell lines. Hemagglutinin or neuraminidase protein binding alone is not sufficient to induce IFIT1 expression. Surprisingly, the expression patterns of IFIT1 in response to H9N2 virus and viral particles in the two cell lines were opposite, and production kinetics of IFN-α/β also differed. An additional finding was that induction of IFIT1 in response to H9N2 virus infection or viral particle inoculation was more sensitive in HUVECs than in BEAS-2Bs. Our data offers new insight into the innate immune response of endothelial cells to H9N2 virus infection.

#endothelial cells #H9N2 virus #IFIT1 expression pattern #IFN-α/β

참고문헌 (57)

참고문헌 신청

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Broz, P. / 2013 / Newly described pattern recognition receptors team up against intracellular pathogens / Nat. Rev. Immunol. 13:551~565

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