Development of a loop-mediated isothermal amplification assay for living modified canola GT73

Yoon A-Mi; Kim Il Ryong; Lim Hye Song; Lee Jung Ro; Choi Wonkyun

doi:10.1007/s11816-022-00774-w

추천

검색

자료유형: 학술저널

저자정보: Yoon A-Mi (LMO Team National Institute of Ecology (NIE) Seocheon 33657 Republic of Korea) Kim Il Ryong (LMO Team National Institute of Ecology (NIE) Seocheon 33657 Republic of Korea) Lim Hye Song (LMO Team National Institute of Ecology (NIE) Seocheon 33657 Republic of Korea) Lee Jung Ro (LMO Team National Institute of Ecology (NIE) Seocheon 33657 Republic of Korea) Choi Wonkyun (LMO Team National Institute of Ecology (NIE) Seocheon 33657 Republic of Korea)

저널정보: 한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제16권 제4호

발행연도: 2022.8

수록면: 479 - 486 (8page)

DOI: 10.1007/s11816-022-00774-w

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Loop-mediated Isothermal Amplification (LAMP) has been developed to amplify target genes of Living Modified (LM) crops using a simple, rapid, cost-effective, and highly specific process under isothermal conditions. PCR-based detection methods were intensively developed to identify LM crops. However, these methods require expensive laboratory equipment and skilled researchers. This study designed six specific primers for isothermal amplification of LM canola GT73 by Bst DNA polymerase. The reaction condition was simple isothermal amplification in 63°C amplification for 30?min and 80°C inactivation for 2?min. The detection limit of newly developed LAMP assays was approximately 0.8?ng of genomic DNA. To confirm the specificity of the LAMP method, we tested 10 certified reference materials of LM canola, two wild relative species samples (Brassica juncea), and a GT73 canola volunteer. As a result, the LAMP method could specifically detect only the GT73 reference material and GT73 volunteer. We performed LAMP assays using twenty LM canola volunteer samples collected in 2017 in South Korea to verify LAMP specificity. The newly developed LAMP method for GT73 is applicable for LM canola DNA detection.

#Living modified organisms?· LMO?· GT73?· LAMP?· Canola

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!