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This study was to investigate the inhibitory activity of oxidation and acetylcholinesterase using acetone, hexane, ethyl acetate, hot water and ethanol extracts of mycelia obtained from submerged culture from Pleurotus eryngii (DC. ex Fr.) Quel. var. ferulae Lanzi for function tea development. Among various solvent extracts on the oxidation inhibitory activity, the ethanol extract obtained 66.18% of the maximum inhibitory activity. In the case of acetylcholinesterase inhibitory activity, the hot water extract obtained 13.24% of the maximum inhibitory activity. The maximum inhibitory activity of acetylcholinesterase was obtained 17.36% at 40℃ of extract temperature. The maximum inhibitory activity of oxidation was obtained 63.28% at 60℃. The oxidation inhibitory activity was increased from 50.63 to 60.17% when extract time was increased from 2hr to 4hr at 60℃. On the other hand, in the case of over 6hr of extract time, it was decreased to 55.43% at 12hr of extract time. Thermal and pH of acetylcholinesterase inhibitor were stable at 10-30℃ and pH 5.0 to 9.0. As a result, it is considered that this pretreatment effect is effective in promoting inhibitory activity of acetylcholinesterase and oxidation using P. eryngii (DC. ex Fr.) Quel. var. ferulae Lanzi mycelia.
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