지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.12
- 수록면
- 409 - 421 (13page)
- DOI
- 10.1007/s13206-022-00065-0
이용수
초록· 키워드
오류제보하기
In recent decades “saliva” has emerged as an important non-invasive biofluid for diagnostic purposes in both human and animal health sectors. However, with the rapid evolution of molecular detection technologies, the limitation has been the lack of an efficient method for the facile amplification of target RNA from such a complex matrix. Herein, we demonstrate the novel application of hydrogel microparticles of primer-immobilized networks (PIN) for direct quantitative reverse transcription PCR (dirRT-qPCR) of viral RNA from saliva samples without prior RNA purification. Each of these highly porous PIN particles operates as an independent reactor. They filter in micro-volumes of the analyte solution. Viral RNA is captured and converted to complementary DNA (cDNA) through the RT step using covalently incorporated RT primers. The PIN with cDNA of the viral target will be ready for subsequent highly specific qPCR. Preceded by heat-treatment for viral lysis, we were able to conduct PIN dirRT-qPCR with 95% efficiency of the matrix (M) gene for influenza A virus (IAV) and 5’ untranslated region (5’ UTR) for chicken coronavirus spiked into saliva samples. The addition of reverse transcriptase enzyme (RTase) and 10% dilution of the matrix improved the assay sensitivity considerably. PIN particles’ compatibility with microfluidic PCR chip technology has significantly reduced total sample processing time to 50 min, instead of an average of 120 min that are normally used by other assays. We anticipate this technology will be useful for other viral RNA targets by changing the incorporated RT primer sequences and can be adapted for onsite diagnostics.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of Hydrogel Microparticle based RT-qPCR for Advanced Detection of BCR-ABL1 Transcripts
BioChip Journal
2019 .01
-
Highly Multiplexed Molecular Diagnosis with Particles of Real-time PCR
한국생물공학회 학술대회
2020 .06
-
RNA Therapy: Current Status and Future Potential
한국생물공학회 학술대회
2021 .10
-
Development of Universal Primers for Use in RT-qPCR and RT-RPA Targeting SARS-CoV-2 Genes
한국생물공학회 학술대회
2023 .10
-
RNA therapy: rich history, various applications and unlimited future prospects
Experimental and Molecular Medicine
2022 .04
-
Double Controlled Release of Therapeutic RNA Modules through Injectable DNA-RNA Hybrid Hydrogel
한국고분자학회 학술대회 연구논문 초록집
2021 .04
-
Rapid Subtyping and Pathotyping of Avian Influenza Virus using Chip-based RT-PCR
BioChip Journal
2019 .01
-
Molecular insights into regulatory RNAs in the cellular machinery
Experimental and Molecular Medicine
2024 .06
-
Pathogenic diversity of RNA variants and RNA variation-associated factors in cancer development
Experimental and Molecular Medicine
2020 .01
-
PWR core calculation based on pin-cell homogenization in three-dimensional pin-by-pin geometry
Nuclear Engineering and Technology
2024 .06
-
아이핀 대량 부정발급 사고에 대한 개선방법 연구
디지털산업정보학회논문지
2015 .01
-
Crosstalk between RNA silencing and RNA quality control in plants
BMB Reports
2023 .06
-
Inhibition of Influenza A Virus Infection by Disrupting Viral Membrane Using a Nanoparticle
한국생물공학회 학술대회
2017 .04
-
Target-specific Signal Amplification Reaction for Real-time Analysis of RNA
한국생물공학회 학술대회
2020 .06
-
POCT Detection of?14 Respiratory Viruses Using Multiplex RT-PCR
BioChip Journal
2021 .12
-
SF-qPCR: Strand Displacement-Based Fast Quantitative Polymerase Chain Reaction
BioChip Journal
2022 .03
-
High performance 3D pin-by-pin neutron diffusion calculation based on 2D/1D decoupling method for accurate pin power estimation
Nuclear Engineering and Technology
2021 .11
-
Optimal design of synthetic circular RNAs
Experimental and Molecular Medicine
2024 .06
-
A pin to Multi-pin Calibration 시 설비 검증 방법
한국정밀공학회 학술발표대회 논문집
2015 .05
-
유전 알고리즘을 활용한 RNA 설계 방법론
한국정보과학회 학술발표논문집
2016 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0