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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 333 - 340 (8page)
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초록· 키워드
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Frequent outbreaks of avian influenza viruses (AIV), which are influenza A viruses, result in heavy economic and national damages. However, current diagnostic methods require about 5-7 days for AIV to be confirmed, which allows enough time for the widespread dissemination of the virus. Therefore, there is a need for rapid and accurate diagnosis of AIV. Here, we developed a diagnostic method using chip- based quantitative reverse transcription-polymerase chain reaction (RT-qPCR) for the rapid identification of the major subtypes H5, H7, and H9 of AIV that influence the pathogenicity of the virus. Specific primer pairs and probes were designed to target the highly conserved Matrix (M) gene regions in influenza A viruses. Specific primer pairs and probes for subtyping were developed by targeting the conserved sequences in the haemagglutinin (HA) gene specific to each subtype. For pathotyping, specific primer pairs and probes for the highly pathogenic avian influenza viruses (HPAIV) were generated by targeting the HA cleavage site, which is highly related to the pathogenicity of the virus. Using the primer pairs and probe sets, we synthesized the major sequence of each type and constructed a standard curve to confirm their detection limit. In addition, we used a chip-based RT-qPCR method to test the samples collected from the faeces of wild birds, cloacal and oropharyngeal swab samples of infected chicken, and allantoic fluids samples incubated with inoculated eggs. As a result, AIV was accurately detected in all samples. Our study shows that by using a single DNA chip in a portable chip- based RT-qPCR device and specific primer pairs and probes, rapid and accurate AIV detection and subtyping and pathotyping is possible, which will reduce the spread of the virus, thereby reducing the economic and national damages.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Hause, B. M. / 2013 / Isolation of a novel swine influenza virus from Oklahoma in 2011 which is distantly related to Human Influenza C viruses / PLoS Pathog 9:e1003176
Perdue, M.L. / 2005 / Public health risk from avian influenza viruses / Avian Dis 49:317~327
Senne, D. / 1996 / Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential / Avian Dis 40:425~437
Koopmans, M. / 2004 / Transmission of H7N7 avian influenza A virus to human beings during a large outbreak in commercial poultry farms in the Netherlands / Lancet 363:587~593
Swayne, D. E. / 2016 / Influenza A virus / John Wiley & Sons, Inc :1~30
Perdue, M.L. / 2005 / Public health risk from avian influenza viruses / Avian Dis 49:317~327
Senne, D. / 1996 / Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential / Avian Dis 40:425~437
Koopmans, M. / 2004 / Transmission of H7N7 avian influenza A virus to human beings during a large outbreak in commercial poultry farms in the Netherlands / Lancet 363:587~593
Swayne, D. E. / 2016 / Influenza A virus / John Wiley & Sons, Inc :1~30
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