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논문 기본 정보

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학술저널
저자정보
천성빈 (서울대학교병원 응급의학과) Min Ji Lee (Department of Emergency Medicine CHA Bundang Medical Center Seongnam 13496 Korea) 오원섭 (강원대학교 의학전문대학원 내과학교실) 박예진 (Department of Emergency Medicine, CHA Bundang Medical Center, Seongnam 13496) 권중명 (Department of Critical Care and Emergency Medicine Mediplex Sejong Hospital Incheon 21080 Korea) 김규석 (Department of Emergency Medicine CHA Bundang Medical Center Seongnam 13496)
저널정보
대한약리학회 The Korean Journal of Physiology & Pharmacology The Korean Journal of Physiology & Pharmacology 제26권 제3호
발행연도
2022.5
수록면
195 - 205 (11page)
DOI
10.4196/kjpp.2022.26.3.195

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Determining blood loss [100% ? RBV (%)] is challenging in the management of haemorrhagic shock. We derived an equation estimating RBV (%) via serial haematocrits (Hct1, Hct2) by fixing infused crystalloid fluid volume (N) as [0.015 × body weight (g)]. Then, we validated it in vivo. Mathematically, the following estimation equation was derived: RBV (%) = 24k / [(Hct1 / Hct2) ? 1]. For validation, nonongoing haemorrhagic shock was induced in Sprague?Dawley rats by withdrawing 20.0%?60.0% of their total blood volume (TBV) in 5.0% intervals (n = 9). Hct1 was checked after 10 min and normal saline N cc was infused over 10 min. Hct2 was checked five minutes later. We applied a linear equation to explain RBV (%) with 1 / [(Hct1 / Hct2) ? 1]. Seven rats losing 30.0%?60.0% of their TBV suffered shock persistently. For them, RBV (%) was updated as 5.67 / [(Hct1 / Hct2) ? 1] + 32.8 (95% confidence interval [CI] of the slope: 3.14?8.21, p = 0.002, R2 = 0.87). On a Bland-Altman plot, the difference between the estimated and actual RBV was 0.00 ± 4.03%; the 95% CIs of the limits of agreements were included within the pre-determined criterion of validation (< 20%). For rats suffering from persistent, non-ongoing haemorrhagic shock, we derived and validated a simple equation estimating RBV (%). This enables the calculation of blood loss via information on serial haematocrits under a fixed N. Clinical validation is required before utilisation for emergency care of haemorrhagic shock.

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