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논문 기본 정보

자료유형
학술저널
저자정보
Choi Hyo-Jin (Department of Bioenergy Science and Technology and Department of Integrative Food Bioscience and Bi) Jin Yong-Su (Department of Food Science and Human Nutrition and Carl R. Woese Institute for Genomic Biology Univ) 이원흥 (전남대학교)
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제32권 제1호
발행연도
2022.1
수록면
117 - 125 (9page)
DOI
10.4014/jmb.2111.11047

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Until recently, four types of cellobiose-fermenting Saccharomyces cerevisiae strains have been developed by introduction of a cellobiose metabolic pathway based on either intracellular β- glucosidase (GH1-1) or cellobiose phosphorylase (CBP), along with either an energy-consuming active cellodextrin transporter (CDT-1) or a non-energy-consuming passive cellodextrin facilitator (CDT-2). In this study, the ethanol production performance of two cellobiose-fermenting S. cerevisiae strains expressing mutant CDT-2 (N306I) with GH1-1 or CBP were compared with two cellobiose-fermenting S. cerevisiae strains expressing mutant CDT-1 (F213L) with GH1-1 or CBP in the simultaneous saccharification and fermentation (SSF) of cellulose under various conditions. It was found that, regardless of the SSF conditions, the phosphorolytic cellobiose-fermenting S. cerevisiae expressing mutant CDT-2 with CBP showed the best ethanol production among the four strains. In addition, during SSF contaminated by lactic acid bacteria, the phosphorolytic cellobiosefermenting S. cerevisiae expressing mutant CDT-2 with CBP showed the highest ethanol production and the lowest lactate formation compared with those of other strains, such as the hydrolytic cellobiose-fermenting S. cerevisiae expressing mutant CDT-1 with GH1-1, and the glucosefermenting S. cerevisiae with extracellular β-glucosidase. These results suggest that the cellobiosefermenting yeast strain exhibiting low energy consumption can enhance the efficiency of the SSF of cellulosic biomass.

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