Hematologic evaluation of peripheral blood in Sprague Dawley rats by chronic exposure to aluminum chloride (AlCl₃)

Alba Rita Valenzuela-Briseño; Jeanette Guadalupe Arredondo-Damian; Antonio Rascón-Careaga; Humberto stiazaran-Garcia; Agustín Gómez-Álvarez; Reynaldo Esquivel-Gónzalez; Roberto Carlos Carrillo-Torres; Enrique Álvarez-Ramos; Rafael Canett-Romero; Leticia García-Rico; Alejandro Monserrat García-Alegría

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저자정보: Alba Rita Valenzuela-Briseño (University of Sonora) Jeanette Guadalupe Arredondo-Damian (University of Sonora) Antonio Rascón-Careaga (University of Sonora) Humberto stiazaran-Garcia (University of Sonora) Agustín Gómez-Álvarez (University of Sonora) Reynaldo Esquivel-Gónzalez (University of Sonora) Roberto Carlos Carrillo-Torres (University of Sonora) Enrique Álvarez-Ramos (University of Sonora) Rafael Canett-Romero (University of Sonora) Leticia García-Rico (Government organization in Hermosillo) Alejandro Monserrat García-Alegría (University of Sonora)

저널정보: 환경독성보건학회 Environmental Analysis Health and Toxicology Environmental Analysis Health and Toxicology Vol.37 No.4

발행연도: 2022.12

수록면: 79 - 93 (15page)

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This study aimed to evaluate whether aluminum chloride (AlCl₃) causes hematological changes in the peripheral blood of Sprague–Dawley (SD) rats. Five groups of female SD rats were intragastrically administered with 4 different concentrations of AlCl₃ for 5 days a week for a total of 90 days. The aluminum concentration was determined via graphite furnace atomic absorption spectroscopy. Analysis of serum iron-kinetic profiles, blood cytometry outcomes, and blood smears of the blood samples. Scanning electron microscopy (SEM) and Raman spectroscopy were used to search for structural and ultrastructural changes, respectively. Blood aluminum concentration ranged 12.38-16.24 μg/L with no significant difference between experimental treatments. At the AlCl₃ concentration of 40 mg Al/kg bw of rats/day, the mean ferritin value in the serum iron kinetic profile was 29.81±6.1 ng/mL, and this value showed a significant difference between experimental treatments. Blood cytometry revealed that there were 6.45-7.11×10<SUP>6</SUP> cells/μL erythrocytes, 8.91-9.32×10³ cells/μL leukocytes, and 477.2-736.3×10³ cells/μL platelets along with a hemoglobin of 37.38-41.93 g/dL and hematocrit level of 37.38-41.93%; the experimental treatments showed no significant differences. Erythrocyte structural analysis using SEM showed no differences between experimental treatments, whereas ultrastructural evaluation using Raman spectroscopy made it possible to identify the following bands: 741, 1123, 1350, 1578, and 1618 cm<SUP>−1</SUP>, which were respectively associated with the following vibrational modes and compounds: vibration of the tryptophan ring, asymmetric C-O-C stretching of glucose, C-H curve of tryptophan, C=C stretching of the heme group, and C-N stretching of the heme group, with no significant differences between experimental treatments. Therefore, AlCl₃ administration does not induce ultrastructural changes in the erythrocyte membrane. This study revealed that serum ferritin concentration was the only parameter affected by AlCl₃ exposure at 40 mg of Al/kg bw of rats/day.

#hematological evaluation #aluminum chloride #Sprague Dawley rats

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