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논문 기본 정보

자료유형
학술대회자료
저자정보
Min Gu Park (Seoul National University) Jae Young Choi (Seoul National University) Dong Hwan Park (Seoul National University) Minghui Wang (Seoul National University) Sang Hee Kim (Seoul National University) Ho Yeon Lee (Seoul National University) Yeon Ho Je (Seoul National University)
저널정보
한국농약과학회 한국농약과학회 학술발표대회 논문집 2022년 한국농약과학회 임시총회 및 추계학술발표회
발행연도
2022.11
수록면
67 - 77 (11page)

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초록· 키워드

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Bacillus thuringiensis (Bt) has been widely recognized for its high potential in the control of lepidopteran pests. However, resistance to Bt is a major concern associated with Spodoptera spp. (Noctuidae) and Plutella xylostella (Plutellidae). For efficient control of Noctuidae and Plutellidae, novel Bt strains which have high toxcitiy and a broad host range are needed. To develop novel Bt strains as used for bio-insecticides, the Bt IMBL-B9 with high toxicity against Spodoptera exigua, Spodoptera frugiperda and P. xylostella was isolated and characterized. The Bt kusrstaki IMBL-B9 strain produced bipuramidal and cuboidal crystals consisting of cry toxins with molecular weights of 130 and 65 kDa, respectively. This strain harbors eight crystal protein genes in total, including cry1Ea and one vegetative insecticidal protein gene. The median lethal concentration (LC50) values of IMBL-B9 against S. exigua and S. frugiperda were 21.8-and 19.3-fold lower than those of the Bt kusrstaki strain, and 5.6-and 4.9-fold lower than those of Bt aizawai strain, respectively. To evaluate the insecticidal activity of Cry proteins from IMBL-B9, cry gene-sourced recombinant Bt strains were constructed. Theses strains have insecticidal activity and synergic action against lepidopteran pests. To increase the transformation efficiency and the stability of cry toxins productions of recombinant Bt strains, a sporulation deficient engineered Bt mutant system were established. As a result, Cry toxin productivity and stability from the improved Bt strain were increased.

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