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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한백신학회 Clinical and Experimental Vaccine Research Clinical and Experimental Vaccine Research Vol.9 No.1
- 발행연도
- 2020.1
- 수록면
- 48 - 55 (8page)
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초록· 키워드
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Purpose: Most cell culture processes for viral vaccine production are mainly based on adherent cell culture systems using serum, which are associated with expensive and labor-intensive processes to produce large amounts of viral vaccine strains. In this study, we investigated whether Vero cells could be grown in serum-free and shaking suspension conditions. Furthermore, we assessed the ability of the Vero cell suspension culture system to produce adenovirus type 5 (Ad5), compared to that of the adhesive Vero cell culture system. Materials and Methods: We tested the feasibility of commercial serum-free media for Vero cell culture. For the adaptation of Vero cells in suspension culture, adhesive Vero cells were added in the early phase of shaking suspension culture, and 50 days after shaking suspension culture, suspension-adapted Vero cells were subcultured continuously. To assess the virus production ability of Vero cells in suspension, the cells were infected with Ad5-green fluorescent protein and evaluated based on their fluorescence intensity. Results: The Vero cells grown in OptiPRO serum-free medium showed no changes in morphology and growth rate, but MRC-5 and FRhk-4 cells showed morphological changes and decreased growth rate, respectively. The Vero cells were well adapted to the suspension culture system. The Vero cells in suspension showed a better Ad5 production ability than the adherent Vero cells. Conclusion: Vero cells can be grown in OptiPRO serum-free medium. Further, our suspension culture-adapted Vero cells may be suitable to produce viral vaccine strains due to their high ability to produce viruses such as Ad5.
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참고문헌 (15)
참고문헌 신청
Barrett PN / 2009 / Vero cell platform in vaccine production : moving towards cell culture-based viral vaccines / Expert Rev Vaccines 8:607~618
Genzel Y / 2015 / Designing cell lines for viral vaccine production : where do we stand / Biotechnol J 10:728~740
Levenbook IS / 1984 / Tumorigenicity of Vero cells / J Biol Stand 12:391~398
Merten OW / 2002 / Development of serum-free media for cell growth and production of viruses/viral vaccines: safety issues of animal products used in serum-free media / Dev Biol (Basel) 111:233~257
Chen A / 2011 / Serum-free microcarrier based production of replication deficient influenza vaccine candidate virus lacking NS1 using Vero cells / BMC Biotechnol 11:81
Genzel Y / 2015 / Designing cell lines for viral vaccine production : where do we stand / Biotechnol J 10:728~740
Levenbook IS / 1984 / Tumorigenicity of Vero cells / J Biol Stand 12:391~398
Merten OW / 2002 / Development of serum-free media for cell growth and production of viruses/viral vaccines: safety issues of animal products used in serum-free media / Dev Biol (Basel) 111:233~257
Chen A / 2011 / Serum-free microcarrier based production of replication deficient influenza vaccine candidate virus lacking NS1 using Vero cells / BMC Biotechnol 11:81
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