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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.11
- 수록면
- 31 - 39 (9page)
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초록· 키워드
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A threat to living organisms resulting from climate change on this planet leads to growing concern about the environment. One of these parts was Agriculture originated organic pollutants, known as a group of environmental contaminants. These contaminants cause many negative effects and even death on living organisms including aquatic vertebrates. But, many toxicological studies were still focused on their viewpoint only on the death problem. In this phenomenon, we tried to explore the possible toxicological effects of EPN on the early developmental stage of aquatic vertebrates using zebrafish embryos. In the acute toxicity test of AB strain zebrafish embryo, both pericardial and yolk sac edemas were observed at 500 ppb EPN treated group. Moreover, the presence of 250 ppb EPA makes zebrafish embryo obtain hematoma near the heart. To confirm phenotype of zebrafish more clearly, three different transgenic line were used including Tg[fli1a:EGFP], Tg[cmlc2:EGFP], and Tg[gata1:GFP]. In transgenic zebrafish, the malformation of the embryos was still observed. Especially, in the fluorescent image of Tg[cmlc2:EGFP], the formation of the heart seemed to be disturbed seriously. In the real-time qPCR data, the level of the transcripts related to early-stage heart formation, nppa, and myl7, were up and down-regulated to 2.0 and 0.26-fold respectively. In addition, the heart rate of the embryo was decreased at 250 and 500 ppb. Taking all these results together, EPN has the possibility of negative effects on the early-stage development of aquatic vertebrates including hematoma, malformation of the heart, and abnormal development of the internal circulatory system. Therefore, continuous monitoring and proper management are required.
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UCI(KEPA) : I410-ECN-0101-2022-523-000212835