지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 355 - 365 (11page)
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Brassica rapa cysteine protease 3 (BrCP3) is an endopeptidase member of C1A family (papain-like) and CA clan of cysteine protease. Members of papain-like cysteine protease (CP) have emerged to be key enzymes involved in cell death in response to biotic and environmental stresses. Recent progress of CP research features its diverse and overlapping role in basal and effector-triggered immunity. This study was carried out to characterize BrCP3 gene in Chinese cabbage and explore its function in rice – Xanthomonas oryzae pv. oryzae pathosystem. Phylogenetic analysis and BLASTP search of its amino acid sequence to the Arabidopsis Protein TAIR 10 database identified BrCP3 as the closest ortholog of Arabidopsis RD21a. The full-length cDNA of BrCP3 was cloned and transformed into the genome of japonica rice cv. ‘Gopum’. Constitutive overexpression of the transgene is driven by Ubi-1 promoter in pFLCIII vector. Regenerated single copy overexpression rice lines were inoculated with three Xoo Korean isolates. Regulation of rice resistance through cysteine protease activity is evident in the overexpression lines which showed enhanced immunity to three isolates as depicted by reduced lesion length compared with the wild type. Our initial results implicate an interesting biological function of BrCP3 in rice system that has never been reported before.
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참고문헌 (38)
참고문헌 신청
Sailila E Abdula / 2013 / Development and Identification of Transgenic Rice Lines with Abiotic Stress Tolerance by using a Full-length Overexpressor Gene Hunting System / Plant Breeding and Biotechnology 1(1):33~48
Adam Z / 2002 / Cutting edge of chloroplast proteolysis / Trends Plant Sci 7:451~456
Adhikari TB / 1995 / Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia / Appl. Environ. Microbiol 61:966~971
Beers EP / 2004 / The S8 serine, C1A cysteine and A1 aspartic protease families in Arabidopsis / Phytochemistry 65:43~58
Bernoux M / 2008 / RD19, an Arabidopsis cysteine protease required for RRS1-R-mediated resistance, is relocalized to the nucleus by the Ralstonia solanacearum PopP2 effector / Plant Cell 20:2252~2264
Adam Z / 2002 / Cutting edge of chloroplast proteolysis / Trends Plant Sci 7:451~456
Adhikari TB / 1995 / Genetic diversity of Xanthomonas oryzae pv. oryzae in Asia / Appl. Environ. Microbiol 61:966~971
Beers EP / 2004 / The S8 serine, C1A cysteine and A1 aspartic protease families in Arabidopsis / Phytochemistry 65:43~58
Bernoux M / 2008 / RD19, an Arabidopsis cysteine protease required for RRS1-R-mediated resistance, is relocalized to the nucleus by the Ralstonia solanacearum PopP2 effector / Plant Cell 20:2252~2264
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