지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 33 - 48 (16page)
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The latest report on the draft genome of Brassica rapa sequence has been published. To elucidate the functions of these genes and to efficiently search for agriculturally useful genes, a Full-length cDNA Over-eXpressor (FOX) gene hunting system was used. The FOX library from Chinese cabbage was introduced into rice via Agrobacterium-mediated transformation. Approximately 1,150 FOX-rice lines were generated. Genomic PCR analysis indicated that the average length of FL-cDNAs introduced into individual lines was 900~1,200 bp. Basic Local Alignment System Tool (BLAST) analysis of the FL-cDNA genes revealed that 35.5% have unknown function. Most of the randomly selected transgenic rice lines showed overexpression (92%) of these genes relative to the wild-type Gopum. Moreover, 94% of the 850 transgenic rice lines were moderately tolerant (slightly yellow) to cold and 9 lines were tolerant (seedlings were light green). Morphological evaluation of the transgenic rice lines showed minimal phenotypic alteration (12%). Approximately 25.1% and 22% of the plants were significantly ahead in the days to heading and had elevated chlorophyll content, respectively. Other agronomic traits such as filled grains, number of tiller, panicle length, and culm and plant height were relatively less variable among the transgenic lines. These results provide a resource for defining genes that are associated with tolerance in transgenic rice lines.
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참고문헌 (42)
참고문헌 신청
Abe H / 2011 / Development of full-length cDNAs from chinese cabbage (Brassica rapa Subsp. pekinensis) and identification of marker genes for defence / DNA Research 18:277~289
Alexandrov NN / 2009 / Insights into corn genes derived from large-scale cDNA sequencing / Plant Mol. Biol 69:179~194
Alexandrov NN / 2006 / Features of Arabidopsis genes and genome discovered using full-length cDNAs / Plant Mol. Biol 60:69~85
An G / 2005 / Reverse genetic approaches for functional genomics of rice / Plant Mol. Biol 59:111~123
Cheng C / 2006 / Epigenetic regulation of the rice retrotransposon Tos17 / Mol. Genet. Genomics 276:378~390
Alexandrov NN / 2009 / Insights into corn genes derived from large-scale cDNA sequencing / Plant Mol. Biol 69:179~194
Alexandrov NN / 2006 / Features of Arabidopsis genes and genome discovered using full-length cDNAs / Plant Mol. Biol 60:69~85
An G / 2005 / Reverse genetic approaches for functional genomics of rice / Plant Mol. Biol 59:111~123
Cheng C / 2006 / Epigenetic regulation of the rice retrotransposon Tos17 / Mol. Genet. Genomics 276:378~390
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