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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 274 - 283 (10page)
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Hemangioblasts or blood islands only arise in early development thereby the sources to obtain these bi-potential cells are limited. While previous studies have isolated both lineages in vitro through the hemangioblast, derivation efficiency was rather low due to cellular damage attributed by enzyme usage and fluorescent activated cell sorting (FACS). This study focused on avoiding the use of damaging factors in the derivation of endothelial cells (ECs). Single cell H9-human embryonic stem cells (hESCs) were obtained by using a mild dissociation protocol then human embryoid body (hEB) formation was performed under hemangioblast differentiation conditions. The hEBs were subjected to a two-stage cytokine treatment procedure. Subsequent culture of the adhesive cells in day 4 hEBs gave arise to a seemingly pure population of ECs. The hESC-derived ECs were characterized by identifying signature endothelial gene and protein markers as well as testing for in vitro functionality. Furthermore, in vivo functionality was also confirmed by transplanting the cells in hindlimb ischemic murine models. We demonstrate that the genetic change required for EC derivation precedes blast colony formation. Furthermore, cell damage was prevented by abating enzyme usage and FACS, resulting in a high yield of ECs upon adhesion. Under this method, confluent cultures of ECs were obtainable 4 days after hEB formation which is significantly faster than previous protocols.
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등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Kennedy M / 1997 / A common precursor for primitive erythropoiesis and definitive haematopoiesis / Nature 386:488~493
Robertson S / 1999 / Hematopoietic commitment during embryogenesis / Ann N Y Acad Sci 872:9~16
Choi K / 2002 / The hemangioblast: a common progenitor of hematopoietic and endothelial cells / J Hematother Stem Cell Res 11:91~101
Forrai A / 2003 / The hemangioblast--between blood and vessels / Cell Cycle 2:86~90
Bollerot K / 2005 / The embryonic origins of hematopoietic stem cells: a tale of hemangioblast and hemogenic endothelium / APMIS 113:790~803
Robertson S / 1999 / Hematopoietic commitment during embryogenesis / Ann N Y Acad Sci 872:9~16
Choi K / 2002 / The hemangioblast: a common progenitor of hematopoietic and endothelial cells / J Hematother Stem Cell Res 11:91~101
Forrai A / 2003 / The hemangioblast--between blood and vessels / Cell Cycle 2:86~90
Bollerot K / 2005 / The embryonic origins of hematopoietic stem cells: a tale of hemangioblast and hemogenic endothelium / APMIS 113:790~803
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