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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 159 - 166 (8page)
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초록· 키워드
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Sample preconcentration is an important step that increases the accuracy of subsequent detection, especially for samples with extremely low concentrations. Due to the overlap of electrical double layers in a nanofluidic channel, the concentration polarization effect can be generated by applying an electric field. A nonlinear electrokinetic flow is induced, which results in the fast accumulation of proteins in front of the induced ionic depletion zone, the so-called exclusion- enrichment effect. In this way, a protein sample can be driven by electroosmotic flow and accumulated at a specific location. In the present study, a nanofluidic preconcentrator fabricated with the help of junction gap electric breakdown was integrated with microelectrodes for immunoassay. The preconcentration chip for proteins was fabricated using simple standard soft lithography with a polydimethylsiloxane replica. Human galectin-1 proteins from the cell lysate of T24 cells were concentrated and immunoassayed in the proposed microchip. The capability of the proposed microchip for concentrating multiple proteins from cell lysates and immunoassays after preconcentration was demonstrated. Immunosensing was evaluated by measurements of both fluorescence intensities and impedance, which proved the enhancement of preconcentration for immunoassay.
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참고문헌 (40)
참고문헌 신청
Pu, Q. / 2004 / Ion-enrichment and ion-depletion effect of nanochannel structures / Nano Lett 4:1099~1103
Duan, C. / 2013 / Review article: Fabrication of nanofluidic devices / Biomicrofluidics 7:026501
Wang, Y. C. / 2005 / Million-fold preconcentration of proteins and peptides by nanofluidic filter / Anal. Chem 77:4293~4299
Mao, P. / 2005 / Fabrication and characterization of 20 nm planar nanofluidic channels by glass-glass and glass-silicon bonding / Lab Chip 5:837~844
Lee, J. H. / 2008 / Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane / Lab Chip 8:596~601
Duan, C. / 2013 / Review article: Fabrication of nanofluidic devices / Biomicrofluidics 7:026501
Wang, Y. C. / 2005 / Million-fold preconcentration of proteins and peptides by nanofluidic filter / Anal. Chem 77:4293~4299
Mao, P. / 2005 / Fabrication and characterization of 20 nm planar nanofluidic channels by glass-glass and glass-silicon bonding / Lab Chip 5:837~844
Lee, J. H. / 2008 / Multiplexed proteomic sample preconcentration device using surface-patterned ion-selective membrane / Lab Chip 8:596~601
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