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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 195 - 203 (9page)
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초록· 키워드
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Antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleoprotein (NP) were purified from pig serum through two steps: (1) isolation of anti-NP IgG antibodies using magnetic beads with immobilized human SARS CoV-2 NP and (2) fi ltration of anti-spike protein (SP) IgG antibodies using magnetic beads with immobilized human SARS-CoV SP. The enhanced specificity of the purified antibodies to the NP of SARS-CoV-2 was demonstrated using an immunoassay with anti-NP IgG antibodies after the isolation and filtration steps. The binding constants ( K d ) of the purified anti-NP IgG antibodies to the NP of SARS-CoV-2 and the SP of SARS-CoV were estimated using a surface plasmon resonance biosensor (SPR). A competitive assay using the two-step purified anti-NP IgG antibodies from pig serum demonstrated (a) the detection of SARS-CoV-2 in viral fluid and (b) the discrimination of SARS-CoV-2 from SARS-CoV, MERS-CoV, and CoV strain 229E in viral fluids.
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등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
봉지홍 / 2020 / Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors / BioChip Journal 14(4):358~368
Elodie Monchatre-Leroy / 2017 / Identification of Alpha and Beta Coronavirus in Wildlife Species in France: Bats, Rodents, Rabbits, and Hedgehogs / Viruses 9(12):364
Di Wu / 2020 / The SARS-CoV-2 outbreak: What we know / International Journal of Infectious Diseases 94:44~48
Yu Chen / 2020 / Emerging coronaviruses: Genome structure, replication, and pathogenesis / Journal of Medical Virology 92(4):418~423
Xiaoxia Lu / 2020 / SARS-CoV-2 Infection in Children / New England Journal of Medicine 382(17):1663~1665
Elodie Monchatre-Leroy / 2017 / Identification of Alpha and Beta Coronavirus in Wildlife Species in France: Bats, Rodents, Rabbits, and Hedgehogs / Viruses 9(12):364
Di Wu / 2020 / The SARS-CoV-2 outbreak: What we know / International Journal of Infectious Diseases 94:44~48
Yu Chen / 2020 / Emerging coronaviruses: Genome structure, replication, and pathogenesis / Journal of Medical Virology 92(4):418~423
Xiaoxia Lu / 2020 / SARS-CoV-2 Infection in Children / New England Journal of Medicine 382(17):1663~1665
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