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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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Background: Hyperoside, a flavonoid which is mainly found in Hypericum perforatum L., has many biological effects. One of the most important effects is to prevent the oxidative stress induced by reactive oxygen species. However, the molecular mechanisms underlying its effect are not fully understood. Oxidative stress is implicated in the occurrence of various physical diseases. A wide array of enzymatic antioxidant defense systems include NADH: quinone oxidoreductase 1, superoxide dismutase, and heme oxygenase-1 (HO-1). In the present study, the protective effects of hyperoside against hydrogen peroxide-induced oxidative stress in human lens epithelial cells, HLE-B3, were investigated in terms of HO-1 induction. Methods: The protein and mRNA expressions of HO-1 were examined by Western blotting and reverse transcriptase-PCR assays, respectively. To evaluate the ability of hyperoside to activate nuclear factor erythroid 2-related factor 2 (Nrf2), Western blotting and electrophoretic mobility shift assay were performed with nuclear extracts prepared from HLE-B3 cells treated with hyperoside. The activation of extracellular signal-regulated kinase (ERK), the upstream kinase of Nrf2 signaling, was monitored by Western blot analysis. The protective effect of hyperoside in HLE-B3 cells against hydrogen peroxide was performed by MTT assay. Results: Hyperoside increased both the mRNA and protein expression of HO-1 in a time- and dose-dependent manner. In addition, hyperoside elevated the level of of Nrf2 and its antioxidant response element-binding activity, which was modulated by upstream of ERK. Moreover, it activated ERK and restored cell viability which was decreased by hydrogen peroxide. Conclusions: Hyperoside is an effective compound to protect cells against oxidative stress via HO-1 induction.
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참고문헌 (23)
참고문헌 신청
Hu J / 2006 / The effects of natural flavonoids on lipoxygenase-mediated oxidation of compounds with a benzene ring structure: a new possible mechanism of flavonoid anti-chemical carcinogenesis and other toxicities / Int J Toxicol 25:295~301
Naderi GA / 2003 / Anti-oxidant effect of flavonoids on the susceptibility of LDL oxidation / Mol Cell Biochem 246:193~196
Koçyildiz ZC / 2006 / Crataegus tanacetifolia leaf extract prevents L-NAMEinduced hypertension in rats: a morphological study / Phytother Res 20:66~70
Liu Z / 2005 / Protective effects of hyperoside (quercetin-3-o-galactoside) to PC12 cells against cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide / Biomed Pharmacother 59:481~490
Zou Y / 2004 / Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro / J Agric Food Chem 52:5032~5039
Naderi GA / 2003 / Anti-oxidant effect of flavonoids on the susceptibility of LDL oxidation / Mol Cell Biochem 246:193~196
Koçyildiz ZC / 2006 / Crataegus tanacetifolia leaf extract prevents L-NAMEinduced hypertension in rats: a morphological study / Phytother Res 20:66~70
Liu Z / 2005 / Protective effects of hyperoside (quercetin-3-o-galactoside) to PC12 cells against cytotoxicity induced by hydrogen peroxide and tert-butyl hydroperoxide / Biomed Pharmacother 59:481~490
Zou Y / 2004 / Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro / J Agric Food Chem 52:5032~5039
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