Molecular characterization and expression of suppressor of cytokine signaling (SOCS) 1, 2 and 3 under acute hypoxia and reoxygenation in pufferfish, Takifugu fasciatus

Dan Wang; Xin Wen; Xinyu Zhang; Yadong Hu; Xinru Li; Wenxu Zhu; Tao Wang; Shaowu Yin

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자료유형: 학술저널

저자정보: Dan Wang (Nanjing Normal University) Xin Wen (Nanjing Normal University) Xinyu Zhang (Nanjing Normal University) Yadong Hu (Nanjing Normal University) Xinru Li (Nanjing Normal University) Wenxu Zhu (Nanjing Normal University) Tao Wang (Nanjing Normal University) Shaowu Yin (Nanjing Normal University)

저널정보: 한국유전학회 Genes & Genomics Genes & Genomics Vol.40 No.11

발행연도: 2018.1

수록면: 1,225 - 1,235 (11page)

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Hypoxia seriously affects the innate immune system of fish. However, the roles of suppressor of cytokine signaling (SOCS), pivotal anti-inflammatory genes, in response to hypoxia/reoxygenation remain largely unexplored. The primary objective of this study was to elucidate the function of SOCS genes under acute hypoxia and reoxygenation in pufferfish (Takifugu fasciatus). In the present study, SOCS1, 2 and 3 were identified in T. fasciatus referred to as TfSOCS1, 2 and 3. Then, qRTPCR and western blot analysis were employed to assess their expressions at both the mRNA and protein levels. Tissue distribution demonstrated that the three SOCS genes were predominantly distributed in gill, brain and liver. Under hypoxia challenge (1.63 ± 0.2 mg/L DO for 2, 4, 6 and 8 h), the expressions of TfSOCS1 and 3 in brain and liver at the mRNA and protein levels were significantly decreased, while their expressions showed an opposite trend in gill. Different from the expressions of TfSOCS1 and 3, the expression of TfSOCS2 was inhibited in gill, along with its increased expression in brain and liver. After normoxic recovery (7.0 ± 0.3 mg/L of DO for 4 and 12 h), most of TfSOCS genes were significantly altered at R4 (reoxygenation for 4 h) and returned to the normal level at R12 (reoxygenation for 12 h). SOCS genes played vital roles in response to hypoxia/reoxygenation challenge. Our findings greatly strengthened the relation between innate immune and hypoxia stress in T. fasciatus.

참고문헌 (48)

참고문헌 신청

Bai L / 2006 / SOCS3 was induced by hypoxia and suppressed STAT3 phosphorylation in pulmonary arterial smooth muscle cells / Respir Physiol Neurobiol 152:83~91

Bicher HI / 1974 / Brain oxygen autoregulation : a protective reflex to hypoxia? / Microvasc Res 8:291~313

Cheng CH / 2015 / High temperature induces apoptosis and oxidative stress in pufferfish(Takifugu obscurus)blood cells / J Therm Biol 53:172~179

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De SE / 2013 / Overexpression of proinflammatory genes and down-regulation of SOCS-1 in human PTC and in hypoxic BCPAP cells / Biomed Pharmacother 67:7~16

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