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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 16 - 24 (9page)
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초록· 키워드
오류제보하기
Most ginseng cultivars bear red berry and only one cultivar ‘Gumpoong’ (GU) bears golden berry. GU is an elite cultivar bred by pedigree selection from a golden berry landrace (a mixed population) ‘Hwangsook’ (HS). We developed three unique polymorphic markers from complete chloroplast genome sequences of GU and HS. A population of GU showed uniform band amplicon against three chloroplast markers whereas HS population displayed mixed genotypes for both GU and HS. Using the characteristics of mixed genotypes in HS population, we developed a convenient method to differentiate GU and HS population by application of pooled DNA template for PCR analysis (pooling method). The pooling method revealed that the GU pool was identical with GU genotype while the HS pool showed both GU and HS genotype. The pooling method is a cost and time effective method for accurate authentication of both golden berry ginseng cultivars. The method is useful to protect GU products from its tentative counterfeits from seeds to mature plant stages as well as processed root products.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Allen GC / 2006 / A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide / Nat. Protoc 1:2320~2325
최홍일 / 2011 / Development of Reproducible EST-derived SSR Markers and Assessment of Genetic Diversity in Panax ginseng Cultivars and Related Species / Journal of Ginseng Research 35(4):399~412
Choi KT / 2008 / Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer / Acta. Pharm. Sinic 29:1109~1118
Ha W / 2002 / Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD) / J. Agric. Food. Chem 50:1871~1875
Dong Su In / 2005 / Genetic Relationships of Panax Species by RAPD andISSR Analyses / 한국약용작물학회지 13(5):249~253
최홍일 / 2011 / Development of Reproducible EST-derived SSR Markers and Assessment of Genetic Diversity in Panax ginseng Cultivars and Related Species / Journal of Ginseng Research 35(4):399~412
Choi KT / 2008 / Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C A Meyer / Acta. Pharm. Sinic 29:1109~1118
Ha W / 2002 / Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD) / J. Agric. Food. Chem 50:1871~1875
Dong Su In / 2005 / Genetic Relationships of Panax Species by RAPD andISSR Analyses / 한국약용작물학회지 13(5):249~253
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