Berberine Suppresses Fibronectin Expression through Inhibition of c-Jun Phosphorylation in Breast Cancer Cells

정이선; 유다은; 강현구; 유종한; 김석원; 남석진; 이정언; 김상민

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자료유형: 학술저널

저자정보: 정이선 (성균관대학교) 유다은 (성균관대학교) 강현구 (성균관대학교) 유종한 (삼성서울병원) 김석원 (성균관대학교) 남석진 (성균관대학교) 이정언 (삼성서울병원) 김상민 (삼성서울병원)

저널정보: 한국유방암학회 Journal of Breast Cancer Journal of Breast Cancer Vol.21 No.1

발행연도: 2018.1

수록면: 21 - 27 (7page)

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Purpose: The exact mechanism regulating fibronectin (FN) expression in breast cancer cells has not been fully elucidated. In this study, we investigated the pharmacological mechanism of berberine (BBR) with respect to FN expression in triple-negative breast cancer (TNBC) cells. Methods: The clinical significance of FN mRNA expression was analyzed using the Kaplan-Meier plotter database (http://kmplot.com/breast). FN mRNA and protein expression levels were analyzed by real-time polymerase chain reaction and western blotting, respectively. Results: Using publicly available clinical data, we observed that high FN expression was associated with poor prognosis in patients with breast cancer. FN mRNA and protein expression was increased in TNBC cells compared with non-TNBC cells. As expected, recombinant human FN significantly induced cell spreading and adhesion in MDA-MB231 TNBC cells. We also investigated the regulatory mechanism underlying FN expression. Basal levels of FN mRNA and protein expression were downregulated by a specific activator protein-1 (AP-1) inhibitor, SR11302. Interestingly, FN expression in TNBC cells was dose-dependently decreased by BBR treatment. The level of c-Jun phosphorylation was also decreased by BBR treatment. Conclusion: Our findings demonstrate that FN expression is regulated via an AP-1–dependent mechanism, and that BBR suppresses FN expression in TNBC cells through inhibition of AP-1 activity.

참고문헌 (30)

참고문헌 신청

Ikram M / 1975 / A review on the chemical and pharmacological aspects of genus Berberis / Planta Med 28:353~358

Liu Z / 2009 / Berberine induces p53-dependent cell cycle arrest and apoptosis of human osteosarcoma cells by inflicting DNA damage / Mutat Res 662:75~83

Kim JB / 2008 / Berberine inhibits growth of the breast cancer cell lines MCF-7 and MDA-MB-231 / Planta Med 74:39~42

Kim S / 2012 / Berberine suppresses the TPA-induced MMP-1 and MMP-9 expressions through the inhibition of PKC-alpha in breast cancer cells / J Surg Res 176:e21~e29

Li X / 2014 / Berberine hydrochloride IL-8 dependently inhibits invasion and IL-8-independently promotes cell apoptosis in MDA-MB-231 cells / Oncol Rep 32:2777~2788

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