Increased Hypermethylation of Glutathione S-Transferase P1, DNA-Binding Protein Inhibitor, Death Associated Protein Kinase and Paired Box Protein-5 Genes in Triple-Negative Breast Cancer Saudi Females

Hafez, Mohamed M.; Al-Shabanah, Othman A.; Al-Rejaie, Salim S.; Al-Harbi, Naif O.; Hassan, Zeinab K.; Alsheikh, Abdulmalik; Theyab, Abdurrahman I. Al; Aldelemy, Meshan L.; Sayed-Ahmed, Mohamed M.

추천

검색

자료유형: 학술저널

저자정보: Hafez, Mohamed M. (Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University) Al-Shabanah, Othman A. (Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University) Al-Rejaie, Salim S. (Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University) Al-Harbi, Naif O. (Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University) Hassan, Zeinab K. (Zoology Department, College of Science, King Saud University) Alsheikh, Abdulmalik (Department of Pathology, College of Medicine, King Saud University) Theyab, Abdurrahman I. Al (Oncology Division, Department of Internal Medicine, College of Medicine, King Saud University) Aldelemy, Meshan L. (King Abdullah International Medical Research Center) Sayed-Ahmed, Mohamed M. (Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University)

저널정보: 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP Asian Pacific journal of cancer prevention : APJCP 제16권 제2호

발행연도: 2015.1

수록면: 541 - 549 (9page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Triple negative breast cancer (TNBC) is an aggressive subtype of breast cancer (BC) with higher metastatic rate and both local and systemic recurrence compared to non-TNBC. The generation of reactive oxygen species (ROS) secondary to oxidative stress is associated with DNA damage, chromosomal degradation and alterations of both hypermethylation and hypomethylation of DNA. This study concerns differential methylation of promoter regions in specific groups of genes in TNBC and non-TNBC Saudi females in an effort to understand whether epigenetic events might be involved in breast carcinogenesis, and whether they might be used as markers for Saudi BCs. Methylation of glutathione S-transferase P1 (GSTP1), T-cadherin (CDH13), Paired box protein 5 (PAX5), death associated protein kinase (DAPK), twist-related protein (TWIST), DNA-binding protein inhibitor (ID4), High In Normal-1 (HIN-1), cyclin-dependent kinase inhibitor 2A (p16), cyclin D2 and retinoic acid receptor-${\beta}$ ($RAR{\beta}1$) genes was analyzed by methylation specific polymerase chain reaction (MSP) in 200 archival formalin-fixed paraffin embedded BC tissues divided into 3 groups; benign breast tissues (20), TNBC (80) and non-TNBC (100). The relationships between methylation status, and clinical and pathological characteristics of patients and tumors were assessed. Higher frequencies of GSTP1, ID4, TWIST, DAPK, PAX5 and HIN-1 hypermethylation were found in TNBC than in non-TNBC. Hypermethylation of GSTP1, CDH13, ID4, DAPK, HIN-1 and PAX5 increased with tumor grade increasing. Other statistically significant correlations were identified with studied genes. Data from this study suggest that increased hypermethylation of GSTP1, ID4, TWIST, DAPK, PAX5 and HIN-1 genes in TNBC than in non-TNBC can act as useful biomarker for BCs in the Saudi population. The higher frequency of specific hypermethylated genes paralleling tumor grade, size and lymph node involvement suggests contributions to breast cancer initiation and progression.

#Breast cancer #TNBC #Non-TNBC #oxidative stress #DNA methylation #Saudi Arabia

참고문헌 (0)

참고문헌 신청

참고문헌이 DBpia에서 서비스 중이라면, [참고문헌 신청]을 통해 등록해보세요

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!