Effect of PDGF-B Gene-Activated Acellular Matrix and Mesenchymal Stem Cell Transplantation on Full Thickness Skin Burn Wound in Rat Model

Paramasivam Tamilmahan; Maiti Swapan Kumar; Palakkara Sangeetha; Rashmi; Mohan Divya; Manjunthaachar H. V.; Karthik K.; Kumar Naveen

추천

검색

자료유형: 학술저널

저자정보: Paramasivam Tamilmahan (ICAR-Indian Veterinary Research Institute) Maiti Swapan Kumar (ICAR-Indian Veterinary Research Institute) Palakkara Sangeetha (ICAR-Indian Veterinary Research Institute) Rashmi (ICAR-Indian Veterinary Research Institute) Mohan Divya (ICAR-Indian Veterinary Research Institute) Manjunthaachar H. V. (ICAR-Indian Veterinary Research Institute) Karthik K. (ICAR-Indian Veterinary Research Institute) Kumar Naveen (ICAR-Indian Veterinary Research Institute)

저널정보: 한국조직공학과 재생의학회 조직공학과 재생의학 조직공학과 재생의학 제18권 제2호

발행연도: 2021.1

수록면: 235 - 251 (17page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background: Full thickness burn wounds are lack of angiogenesis, cell migration, epithelialisation and finally scar tissue formation. Tissue engineered composite graft can provide sustained release of growth factor and promote the wound healing by cell migration, early angiogenesis and proliferation of extracellular matrix and wound remodeling. The objective of this study was to evaluate the gene embedded (pDNA-platelet-derived growth factor, PDGF-B) porcine acellular urinary bladder matrix with transfected mesenchymal stem cells (rBMSC) on healing of full thickness burn wound in rat model. Methods: Full thickness burn wound of 2 × 2 cm size was created in dorsum of rat model under general anesthesia. Burn wounds were treated with silver sulfadiazine; porcine acellular urinary bladder matrix (PAUBM); PAUBM transfected with pDNA-PDGF-B; PAUBM seeded with rBMSC; PAUBM seeded with rBMSC transfected with pDNA-PDGF-B in groups A, B, C, D and E respectively. The wound healing was assessed based on clinical, macroscopically, immunologically, histopathological and RT-qPCR parameters. Results: Wound was significantly healed in group E and group D with early extracellular matrix deposition, enhanced granulation tissue formation and early angiogenesis compared to all other groups. The immunologic response against porcine acellular matrix showed that PDGF-B gene activated matrix along with stem cell group showed less antibody titer against acellular matrix than other groups in all intervals. PDGF gene activated matrix releasing the PDGF-B and promote the healing of full thickness burn wound with neovascularization and neo tissue formation. PDGF gene also enhances secretion of other growth factors results in PDGF mediated regenerative activities. This was confirmed in RT-qPCR at various time intervals. Conclusion: Gene activated matrix encoded for PDGF-B protein transfected stem cells have been clinically proven for early acceleration of angiogenesis and tissue regeneration in burn wounds in rat models.

#Acellular matrix PDGF-B Gene therapy Stem cell Burn wound

참고문헌 (60)

참고문헌 신청

Herndon DN / 1993 / Growth factors : local and systemic / Arch Surg 128:1227~1233

Gajiwala K / 2004 / Evaluation of lyophilised gamma-irradiated amnion as biological dressing / Cell Tissue Bank 5:73~80

Merguerian PA / 2000 / Acellular bladder matrix allografts in the regeneration of functional bladders : evaluation of large-segment([ 24 cm)substitution in a porcine model / BJU Int 85:894~898

Chen MK / 2001 / Small bowel tissue engineering using small intestinal sub mucosa as a scaffold / J Surg Res 99:352~358

Crapo PM / 2011 / An overview of tissue and whole organ decellularization processes / Biomaterials 32:3233~3243

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!