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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 607 - 624 (18page)
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Background: The delivery of growth factors using a carrier system presents a promising and innovative tool in tissue engineering and dentistry today. Two of the foremost bioactive factors, bone morphogenetic protein-2 and vascular endothelial growth factor (VEGF), are widely applied using a ceramic scaffold. The aim of this study was to determine the use of hydroxyapatite microcarrier (MC) for dual delivery of osteogenic and angiogenic factors to accelerate hard tissue regeneration during the regenerative process. Methods: Two MCs of different sizes were fabricated by emulsification of gelatin and alpha-tricalcium phosphate (α-TCP). The experimental group was divided based on the combination of MC size and growth factors. For investigating the in vitro properties, rat mesenchymal stem cells (rMSCs) were harvested from bone marrow of the femur and tibia. For in vivo experiments, MC with/without growth factors was applied into the standardized, 5-mm diameter defects, which were made bilaterally on the parietal bone of the rat. The animals were allowed to heal for 8 weeks, and samples were harvested and analyzed by micro-computed tomography and histology. Results: Improved proliferation of rat mesenchymal stem cells was observed with VEGF loaded MC. For osteogenic differentiation, dual growth factors delivered by MC showed higher osteogenic gene expression, alkaline phosphatse production and calcium deposition. The in vivo results revealed statistically significant increase in new bone formation when dual growth factors were delivered by MC. Dual growth factors administered on a calcium phosphate matrix showed significantly enhanced osteogenic potential. Conclusion: We propose this system has potential clinical utility in providing solutions for craniofacial bone defects, with the added benefit of early availability.
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참고문헌 (31)
참고문헌 신청
Mourao CF / 2015 / Obtention of injectable platelets rich-fibrin(i-PRF)and its polymerization with bone graft : technical note / Rev Col Bras Cir 42:421~423
Yu WL / 2017 / Enhanced osteogenesis and angiogenesis by mesoporous hydroxyapatite microspheres-derived simvastatin sustained release system for superior bone regeneration / Sci Rep 7:44129
Dickson KF / 1995 / The importance of the blood supply in the healing of tibial fractures / Contemp Orthop 30:489~493
Stegen S / 2015 / Bringing new life to damaged bone : the importance of angiogenesis in bone repair and regeneration / Bone 70:19~27
Vo TN / 2012 / Strategies for controlled delivery of growth factors and cells for bone regeneration / Adv Drug Deliv Rev 64:1292~1309
Yu WL / 2017 / Enhanced osteogenesis and angiogenesis by mesoporous hydroxyapatite microspheres-derived simvastatin sustained release system for superior bone regeneration / Sci Rep 7:44129
Dickson KF / 1995 / The importance of the blood supply in the healing of tibial fractures / Contemp Orthop 30:489~493
Stegen S / 2015 / Bringing new life to damaged bone : the importance of angiogenesis in bone repair and regeneration / Bone 70:19~27
Vo TN / 2012 / Strategies for controlled delivery of growth factors and cells for bone regeneration / Adv Drug Deliv Rev 64:1292~1309
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